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目的:观察菖郁导痰汤对抑郁大鼠模型大脑额叶5-羟色胺受体A2(5-HT2AR)及其mRNA表达的干预,分析抑郁症的发病机制。方法:经Open-Field法[1-3]评分测定评分相近的48只SD普通级大鼠,随机分为4组:空白对照、模型组、菖郁导痰汤3.9 g·kg-1组、氟西汀0.36 mg·kg-1组。每组12只。采用孤养和长期不可预见的慢性应激21 d造成大鼠抑郁症模型,然后开始给药(ig)连续21 d,取脑,免疫组化法检测与5-HT2AR,原位杂交法测5-HT2AR mRNA的表达。结果:与对照组比,模型组5-HT2AR和mRNA的表达数量明显下降,且5-HT2AR/mRNA增高(P<0.01);与模型组比,菖郁导痰汤组5-HT2AR和mRNA表达升高,5-HT2AR/mRNA下降(P<0.01)。结论:抑郁症发病可能与5-HT2AR及其mRNA的表达下降有关;菖郁导痰汤有抗抑郁作用,其抗抑郁作用机制可能是通过上调5-HT2AR及其mRNA的表达。
Objective: To observe the effect of Changyuzhutan Decoction on the expression of 5-HT2AR and its mRNA in the frontal lobe of depressive rats, and to analyze the pathogenesis of depression. Methods: Forty-eight SD normal rats with similar score were measured by Open-Field method. The rats were randomly divided into 4 groups: control group, model group, Changyu Dantang Decoction (3.9g · kg -1) Fluoxetine 0.36 mg · kg-1 group. 12 in each group. The model of depression was induced by solitary and long-term unforeseen chronic stress in rats for 21 days. Then the rats were sacrificed for ig for 21 days continuously. The brain was harvested for immunohistochemistry and 5-HT2AR. -HT2AR mRNA expression. Results: Compared with the control group, the expression of 5-HT2AR and mRNA in the model group was significantly decreased and the expression of 5-HT2AR / mRNA was increased (P <0.01). Compared with the model group, the expression of 5-HT2AR and mRNA Increased, 5-HT2AR / mRNA decreased (P <0.01). Conclusions: The incidence of depression may be related to the decrease of 5-HT2AR and its mRNA expression. Anti-depression effect of Changyuzhutan decoction may be related to the antidepressant effect by up-regulating the expression of 5-HT2AR and its mRNA.