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苹果离体新梢在继代培养过程中，以２０００一３０００ｌｘ光照１０ｈ对增殖、生长较好，而以２０００ｌｘ、１０ｈ效果更为理想；昼夜变温２８℃／２４℃与恒温（２６℃）处理增殖和生长无显著差异，但在昼夜２６℃／２２℃、２４℃／２０℃变温下降低了增殖量与有效新梢数；长富２号增殖和生长以ＭＳ＋ＢＡ１ｍｇ·ｌ￣（－１）＋ＩＢＡ０．１ｍｇ·ｌ￣（－１）＋ＣＨ２００ｍｇ·ｌ￣（－１）效果较好，但依继代次数和新梢状态可在ＢＡ０．７一１．０ｍｇ·ｌ￣（－１），ＩＢＡ（或ＩＡＡ）０．１一０．２ｍｇ·ｌ￣（－１）（Ｈ３０一２００ｍｇ·ｌ￣（－１）范围内变动。
The shoots of apple isolated in vitro were proliferated and grew at 2000-3000lx light for 10h, while the effect was better at 2000lx and 10h. The temperature was 28 ℃ / 24 ℃ and the temperature was 26 ℃, However, the proliferation and the number of effective shoots decreased with the increasing temperature of 26 ℃ / 22 ℃ and 24 ℃ / 20 ℃. The growth and proliferation of Changfu 2 was MS + BA1mg · l ~ (-1) + IBA0. 1mg · l ~ (-1) + CH200mg · l ~ (-1) is better, but the succession times and shoots can be in the range of BA0.7 ~ 1.0mg · l ~ (-1), IBA ) 0.1 a 0.2mg · l ~ (-1) (H30 a 200mg · l ~ (-1) range of changes.