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目的观察异戊酸对非洲爪蟾蝌蚪运动的影响,探讨异戊酸对非洲爪蟾卵母细胞膜上甘氨酸耦联的抑制性氯离子通道的影响及其与异戊酸麻醉样作用的关系。方法对非洲爪蟾的蝌蚪在体观察异戊酸的制动效应;采用微注射法使非洲爪蟾卵母细胞上过度表达α1甘氨酸受体,并通过双电极电压钳技术测量异戊酸对甘氨酸耦联的抑制性氯离子通道功能的影响。结果异戊酸在非洲爪蟾蝌蚪中可产生类麻醉效应,其EC50为(44.8±3.7)mmol/L;Hill系数为4.1±1.2;浓度为5.0、7.5、10、20mmol/L的异戊酸可明显增强甘氨酸诱发的氯离子通道电流强度,增强程度为(19.4±4.9)%、(78.6±5.6)%、(107±27)%、(187±39)%,有显著性差异(P<0.01)。异戊酸可呈浓度依赖性地增加通过非洲爪蟾卵母细胞上甘氨酸耦联抑制性氯离子通道的电流。结论异戊酸对非洲爪蟾蝌蚪具有制动作用,其机制可能与增强对甘氨酸耦联抑制性氯离子通道的电流有关。
OBJECTIVE To investigate the effect of isovaleric acid on the movement of tadpoles in Xenopus laevis and to investigate the effect of isovaleric acid on the inhibitory chloride ion channel of glycine coupling in Xenopus laevis oocytes and its relationship with isovaleric anesthesia. Methods Tadpoles of Xenopus laevis were used to observe the braking effect of isovaleric acid in vivo. The α1 glycine receptor was overexpressed on Xenopus laevis oocytes by microinjection and the effects of isovaleric acid on glycine Effect of coupled inhibitory chloride ion channel function. Results Isovaleric acid could induce anesthetic effects in Xenopus laevis tadpoles with an EC50 of (44.8 ± 3.7) mmol / L and a Hill coefficient of 4.1 ± 1.2. Isovaleric acid concentrations of 5.0, 7.5, 10 and 20 mmol / L (19.4 ± 4.9)%, (78.6 ± 5.6)%, (107 ± 27)% and (187 ± 39)%, respectively, with a significant difference (P < 0.01). Isovaleric acid increased concentration-dependently the current passing through glycine-coupled inhibitory chloride channels on Xenopus oocytes. Conclusion Isovalerate has a braking effect on Xenopus laevis tadpoles, which may be related to the increase of the current of glycine-coupled inhibitory chloride channel.