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以抗禾谷孢囊线虫(Heterodera avenae Wollenweber,cereal cyst nematode,CCN)小麦品系‘E-10’和易感材料‘中国春’为材料,通过人工接种禾谷孢囊线虫二龄幼虫,并在接种线虫后测定根部防御酶苯丙氨酸转氨酶(PAL)和脂氧合酶(LOX)活力变化,研究抗感性材料对线虫侵染后防御酶的响应,以了解作物的抗虫机制.结果发现,‘E-10’和‘中国春’防御酶活力变化存在显著差异,‘E-10’在线虫侵染后6 h,LOX酶活增强,在24 h达到最大值,酶活变化比‘中国春’迅速,暗示‘E-10’启动线虫防御反应更为有效直接.此外,发现小麦在接种线虫后会引起邻近未接种植株根部防御酶活力变化.‘E-10’在接种线虫后12 h,其接触组未接种材料根部PAL酶活增加0.9倍,LOX酶活增加1.1倍,并且‘E-10’未接种植株的酶活变化比‘中国春’更迅速,幅度更剧烈.表明可能有某些气态或挥发性物质参与了小麦防御线虫侵染过程,并且这种现象在抗感材料间差异显著.使用茉莉酸甲酯处理‘E-10’和‘中国春’,小麦根部PAL和LOX酶活并未产生与接触接种线虫材料相一致的变化,表明该气态或挥发性物质并非茉莉酸甲酯.
The second instar larvae of cereal cyst nematode were inoculated by artificial inoculation with Heterodera avenae Wollenweber (cereal cyst nematode) wheat line ’E-10’ and susceptible material ’Chinese Spring’ After inoculated with nematode, the changes of root defense enzyme phenylalanine aminotransferase (PAL) and lipoxygenase (LOX) activity were measured, and the response of anti-sensitive material to defensive enzymes after nematode infection was studied to find out the mechanism of crop insect resistance. There was a significant difference in the activities of defense enzymes between ’E-10’ and ’Chinese Spring’. LOX activity increased at 6 h after infection of ’E-10’ and reached the maximum at 24 h. Spring ’rapidly, suggesting that’ E-10 ’started the nematode defense response more effectively and directly. In addition, it was found that the inoculation of nematodes in wheat caused a change in the activity of defense enzymes in the roots of uninoculated plants.’ E-10 ’ , And the non-inoculated material in the contact group had a 0.9-fold increase in PAL activity and a 1.1-fold increase in LOX activity, and the change in enzyme activity of ’E-10’ unvaccinated plants was more rapid and more violent than that of ’Chinese Spring’ Some gaseous or volatile substances are involved in the defense of the wheat line Process, and this phenomenon has significant differences among the resistant materials. PAL and LOX activities at the roots of wheat did not produce the same changes as those exposed to the nematodes inoculated with methyl jasmonate for ’E-10’ and ’Chinese Spring’ , Indicating that the gaseous or volatile material is not methyl jasmonate.