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本文应用套式PCR检测了156例新生儿肺炎患儿鼻咽部分泌物中的人型支原体(Mh)特异性DNA,其结果:患儿阳性数130例,阳性率833%;而应用人型支原体培养法及单式PCR阳性率均比应用套式PCR为低,提示了临床必须对人型支原体的感染率及致病性引起足够的重视。同时,通过本文研究也提示了临床对于不同的支原体感染可采用不同的抗生素治疗。目前国内对于人型支原体与新生儿肺炎的相关性报道尚未检索到。
In this paper, nested PCR was used to detect Mh-specific DNA in nasopharyngeal secretions from 156 neonates with pneumonia. The results showed that there were 130 positive cases in children, the positive rate was 833% Mycoplasma hominis culture and single-positive rate of PCR were lower than the nested PCR, suggesting that the clinical must pay enough attention to the infection rate and pathogenicity of Mycoplasma hominis. At the same time, through this study also suggests that the clinical application of different antibiotics for different mycoplasma infections. At present, the related reports of Mycoplasma hominis and neonatal pneumonia have not been retrieved yet.