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运用媒介昆虫的研究方法和电镜技术,研究柑桔木虱传播黄龙病的规律和病原在虫体内的分布。结果表明,单只成虫会传病,其指示柑桔苗的发病率高达80％。带“毒”成虫在柑桔苗上传“毒”1～3小时不会传病,5小时以上会传病。1～3龄若虫不会传病,成虫体内携带的病原也不会经卵传到后代。4～5龄若虫会传病,高龄带“毒”若虫羽化的成虫不经再饲“毒”也会传病,高龄若虫携带的病原会跨龄传到成虫。应用电镜除在木虱的唾液腺细胞内观察到病原外,还发现黄龙病的病原体广泛地分布在成虫消化道的滤室、中肠和后肠的细胞内。由于木虱具有选择寄主嫩梢产卵的习性和黄龙病树梢期紊乱,因此,田间病树不但是黄龙病的“毒”源树,而且是其介体昆虫柑桔木虱的虫源树。这些残留的病树和从病树扩散的木虱是黄龙病在田间流行的根源。
Using the research methods of vector insects and electron microscopy techniques, the law of transmission of Huanglongbing by citrus psyllid and the distribution of the pathogen in the parasites were studied. The results showed that only adults could transmit the disease, which indicates that the incidence of citrus seedlings is as high as 80%. With “poisonous” adult upload citrus “poison” 1 to 3 hours will not be transmitted, more than 5 hours will be transmitted. 1 to 3 instar nymphs will not be transmitted, and the pathogen contained in the adult worms will not pass through the egg to future generations. 4 ~ 5 instar nymphs will be transmitted, the elder adults with “poisonous” nymphs become adults without being re-fed “poisonous”, and the pathogen of elder nymphs will be transmitted to adults by age. Electron microscopy in addition to pathogen observed in salivary gland cells of psyllids, but also found that the pathogen of the Huanglong disease widely distributed in the digestive tract of adult digestive tract cells, cells of the midgut and hindgut. Because Psylla had the habit of choosing the tips of young shoots to lay eggs and the treetopsy disorder of Huanglongdao, the diseased trees in the field were not only the “poisonous” source trees of Huanglongbing, but also the insect-source trees of the citrus psyllid . These remaining diseased trees and psyllids that spread from diseased trees are the source of the yellow dragon’s disease in the field.