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以高温厌氧细菌热纤维梭菌(Clostridium thermocellum LQRI和VPI菌株)和嗜热厌氧乙醇菌(Thermoanaerobacterethanolicus X514和39E菌株)为对象,以不同浓度纤维素为底物,分析了热纤维梭菌不同菌株的纤维素降解和酒精生产能力.结果表明,在热纤维梭菌纯培养体系中,LQRI对纤维素的降解能力明显高于VPI,前者约为后者的1.2倍,但两者的酒精生产能力没有显著差异,LQRI在1%纤维素条件下酒精终浓度约为12 mmol/L,VPI约为10 mmol/L.随着底物纤维素浓度的增加(1%、2%、5%),两者对纤维素降解和酒精生产能力均呈明显下降趋势.在热纤维梭菌和嗜热厌氧乙醇菌混合培养体系中,LQRI对纤维素的降解能力明显高于VPI,前者约为后者的1.28～1.58倍.随着底物浓度的增加,两者的纤维素降解率均逐渐下降,但热纤维梭菌对纤维素降解的绝对数值还受到其它混合培养菌种的影响.此外,LQRI+Thermoanaerobacter混合培养体系的酒精生产能力明显高于VPI+Thermoanaerobacter,前者约为后者的1.27～1.77倍.随着底物浓度的增加,混合培养体系酒精生产能力没有明显下降趋势.
The thermophilic anaerobic bacteria Clostridium thermocellum LQRI and VPI strain and Thermoanaerobacterethanolicus X514 and 39E strains were used as the object to analyze the difference of Clostridium thermocellum between different concentrations of cellulose Strains of cellulose degradation and alcohol production capacity.The results showed that in the pure culture system of Clostridium thermocellum, LQRI degradation of cellulose was significantly higher than the VPI, the former is about 1.2 times the latter, but the two alcohol production LQRI had a final alcohol concentration of about 12 mmol / L at 1% cellulose and a VPI of about 10 mmol / L with LQRI concentration increasing (1%, 2%, 5%) with substrate cellulose , Both on cellulose degradation and alcohol production capacity showed a significant downward trend in the hybrid thermophilic thermophilic anaerobic ethanol bacteria culture system LQRI degradation of cellulose was significantly higher than the VPI, the former is about the latter 1.28 ~ 1.58 times higher than that of the control.The cellulase degradation rate of both was gradually decreased with the increase of substrate concentration, but the absolute value of cellulose degradation by Clostridium thermocellum was also affected by other mixed culture strains.In addition, LQRI + Thermoanaerobacter mixed culture The alcohol productivity of the system was significantly higher than that of VPI + Thermoanaerobacter, the former was about 1.27 ~ 1.77 times of that of the latter.With the increase of the substrate concentration, the alcohol production capacity of the mixed culture system showed no obvious declining trend.