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为考察参乌健脑胶囊中人参皂苷Rg1、Rb1、Re、Rd和Rf含量,以三氯甲烷为溶剂回流提取法提取人参中的干扰物质,进一步用水饱和正丁醇超声提取人参总皂苷,甲醇定容后采用高效液相色谱法分析人参皂苷Rb1、Rg1、Re、Rf和Rd的含量。其中,色谱条件为Krm asil C18柱(4.6 mm×250.0 mm,5μm),以乙腈(A)和水(B)系统作为流动相,进行梯度洗脱(0~35 min,19%A;35~55 min,19%A→29%A;55~70 min,29%A;70~100 min,29%A→40%A),流速为1.0 m L/min,检测波长203 nm,柱温30℃。结果显示:参乌健脑胶囊中人参皂苷Re、Rg1、Rf、Rd和Rb1的含量分别为(2.43×10-3)%、(3.98×10-3)%、(4.04×10-3)%、(7.54×10-4)%和(1.78×10-4)%。由此表明,该方法可靠、简洁、快速、灵敏度高,具有色谱柱可反复使用、样品不被破坏、易回收等优点,可用于参乌健脑胶囊中人参皂苷的定量分析。
In order to investigate the content of ginsenosides Rg1, Rb1, Re, Rd and Rf in Shenwujiannao Capsules, the interference substances in ginseng were extracted by refluxing with trichloromethane, and the total ginsenosides, methanol After volume determination, the contents of ginsenosides Rb1, Rg1, Re, Rf and Rd were analyzed by high performance liquid chromatography. The chromatographic conditions were Krm asil C18 column (4.6 mm × 250.0 mm, 5 μm) with gradient elution (0-35 min, 19% A; 35-35 ° C) using acetonitrile (A) and water 55 min, 29% A, 70 min -100 min, 29% A → 40% A) at a flow rate of 1.0 m L / min with a detection wavelength of 203 nm and a column temperature of 30 ℃. The results showed that the content of ginsenoside Re, Rg1, Rf, Rd and Rb1 in Shenwujiannao Capsule were (2.43 × 10-3)%, (3.98 × 10-3)% and (4.04 × 10-3)% respectively, , (7.54 × 10 -4)% and (1.78 × 10 -4)%. The results show that the method is reliable, simple, rapid and sensitive, with the advantages of column reusability, sample damage, easy recovery and so on. It can be used for the quantitative analysis of ginsenosides in Shenwujiannao Capsules.