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目的分析四川省男男同性恋(MSM)人群中未经抗病毒治疗的人类免疫缺陷病毒I型(HIV-1)感染者的耐药突变的流行情况。方法采集成都、绵阳和达州3个地区MSM中未经治疗的HIV-1感染者血样,进行核酸提取、PCR及基因测序,对所得结果登录美国斯坦福大学HIV耐药数据库进行在线分析确定耐药突变情况。结果 77例样本中,11例存在蛋白酶抑制剂次要相关突变,4例存在核苷类逆转录酶抑制剂相关突变,5例存在非核苷类逆转录酶抑制剂相关突变,其中1例潜在耐药。结论四川省MSM中未经治疗的HIV-1感染者少数存在原发耐药相关基因变异,未发现新耐药毒株,原发耐药株仍处于较低流行状态,感染者在抗病毒治疗开始前应定期检测耐药基因突变情况以指导用药。
Objective To analyze the prevalence of drug-resistant mutations in human immunodeficiency virus type 1 (HIV-1) non-antiviral patients among men who have sex with men (MSM) in Sichuan province. Methods Blood samples from untreated HIV-1 infected MSM in Chengdu, Mianyang and Dazhou were collected for nucleic acid extraction, PCR and gene sequencing. The results were registered online at the Stanford HIV drug resistance database to identify drug-resistant mutations Happening. Results Among the 77 samples, there were 11 minor mutations associated with protease inhibitors, 4 mutations associated with nucleoside reverse transcriptase inhibitors and 5 mutations associated with non-nucleoside reverse transcriptase inhibitors, of which 1 had potential resistance medicine. Conclusions The minority of untreated HIV-1 infected MSM patients in Sichuan Province have the variation of primary resistance-related genes, no new drug-resistant strains are found, and the primary drug-resistant strains are still in a low prevalence status. Before the start of regular testing of drug resistance mutations to guide the medication.