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目的:以异硫氰酸-α-萘酯(alpha-naphthylisothi,ANIT)大鼠黄疸模型为载体,探索茯苓多糖退黄作用。方法:Wistar雄性大鼠50只随机分为正常组(10只)和ANIT处理组(40只),2%的ANIT按照6 mL·kg-1ig,48 h后ANIT处理组大鼠随机分为模型对照组及茯苓多糖低、中、高剂量(5,50,500 mg·kg-1),每组10只。模型大鼠每天ig给予生理盐水。茯苓多糖组ig给药1周,实验结束处死全部大鼠,检测大鼠肝功能及实时定量(real-time PCR)方法检测白介素-1β(IL-1β)及肿瘤坏死因子(TNF-α)水平。结果:ANIT处理组大鼠肝功能紊乱,与正常组相比,血清总胆红素(TBil)含量显著升高(P<0.01),为正常组的52.7倍,谷丙转氨酶(ALT)活性显著升高(P<0.01),为正常组的6.31倍,天冬氨酸转氨酶(AST)活性显著升高(P<0.01),为正常组的8.31倍。茯苓多糖退黄疸保肝作用呈剂量依赖型增高,中剂量组退黄有一定作用,高剂量组退黄作用最为显著。ANIT造模组IL-1β,TNF-αmRNA促炎症因子显著升高(P<0.01),分别为正常组的14.24,10.65倍;而模型组IL-4 mRNA表达仅为正常组的0.33(P<0.01);与模型组相比,高剂量茯苓多糖显著抑制IL-1β,TNF-α的mRNA表达(P<0.01),茯苓多糖高剂量组显著提高IL-4 mRNA表达(P<0.01)。结论:高剂量茯苓多糖可能通过免疫调节发挥退黄作用。
Objective: To explore the anti-yellowing effect of tuckahoe polysaccharide with jaundice model of alpha-naphthylisothi (ANIT) rat as carrier. Methods: Fifty Wistar male rats were randomly divided into normal group (n = 10) and ANIT group (n = 40). 2% ANIT group was treated with 6 mL · kg-1 of ANIT for 48 h, The control group and tuckahoe polysaccharide at low, medium and high doses (5,50,500 mg · kg-1), 10 in each group. The model rats were given ig daily with ig. At the end of the experiment, all the rats were sacrificed and the hepatic function and the level of interleukin-1β (IL-1β) and tumor necrosis factor (TNF-α) were detected by real-time PCR . Results: Compared with the normal group, the level of total bilirubin (TBil) in ANIT-treated rats was significantly increased (P <0.01), which was 52.7-fold higher than that in the normal group. The activity of alanine aminotransferase (ALT) (P <0.01). The activity of aspartate aminotransferase (AST) was significantly increased (P <0.01), 8.31 times that of the normal group. Tuckahoe Polysaccharide jaundice liver function in a dose-dependent manner, the middle dose of jaundice has a certain role, high-dose group has the most significant effect of jaundice. The levels of IL-1βand TNF-αmRNA in ANIT model group were significantly increased (P <0.01), which were 14.24 and 10.65 times higher than that in normal group respectively, while the expression of IL-4 mRNA in model group was only 0.33 (P < 0.01). Compared with the model group, high dose of tuckahoe polysaccharide significantly inhibited the mRNA expression of IL-1β and TNF-α (P <0.01), while the high dose of tuckahoe polysaccharide significantly increased the expression of IL-4 mRNA (P <0.01). Conclusion: High-dose tuckahoe polysaccharide may exert its anti-yellowing effect through immune regulation.