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目的:观察益气活血法对胃癌前病变大鼠血清细胞因子的影响。方法:64只SD大鼠随机分为正常对照组8只和造模组56只,采用以MNNG损伤为主的综合法复制大鼠胃癌前病变模型,随机处死10只大鼠经病理确定造模成功后,再将46只造模组大鼠随机分为模型组12只、益气活血高、低剂量组各12只和胃复春组10只,分别给予益气活血方、胃复春混悬液灌胃治疗12周。ELISA法和放射免疫法测定大鼠血清TNF-α、IL-6和IL-8水平。结果:与正常对照组比较,模型组大鼠血清TNF-α、IL-6、IL-8水平较正常组降低,其中IL-8水平明显降低(P<0.01);药物治疗后与模型组比较益气活血高、低剂量组及胃复春组大鼠血清IL-6水平明显降低(P<0.01,P<0.05);胃复春组大鼠血清IL-8水平较益气活血高、低剂量组升高(P<0.05)。结论:益气活血法可改善胃癌前病变大鼠模型胃黏膜组织病理学,调节TNF-α和IL-6、IL-8水平可能是其防治胃癌前病变大鼠的作用机制之一。
Objective: To observe the effect of Yiqi Huoxue Therapy on serum cytokines in precancerous lesions of rats. Methods: Sixty-four Sprague-Dawley rats were randomly divided into normal control group (n = 8) and model group (n = 56). The model of gastric precancerous lesion was established by a comprehensive method including MNNG injury. Ten rats were randomly sacrificed and pathologically confirmed After successful, 46 rats in model group were randomly divided into model group (n = 12), nourishing qi and activating blood high and low dose group (n = 12) and Wei Fu Chun group (n = 10) The suspension was intragastrically treated for 12 weeks. Serum levels of TNF-α, IL-6 and IL-8 were measured by ELISA and radioimmunoassay. Results: Compared with the normal control group, the levels of serum TNF-α, IL-6 and IL-8 in the model group were lower than those in the normal group, and the level of IL-8 in the model group was significantly lower (P <0.01) The level of IL-6 in YiFuHuoxue high, low dose and Weifuchun groups was significantly lower than that in YiFuHuoxue group (P <0.01, P <0.05) Dose group increased (P <0.05). Conclusion: Yiqi Huoxue Therapy can improve gastric mucosal histopathology and regulate the levels of TNF-α, IL-6 and IL-8 in gastric precancerous lesions in rats, which may be one of its mechanisms in preventing gastric precancerous lesions.