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目的调查重庆市不同年龄健康人结膜囊需氧菌种类及数量。方法湿棉拭于33例儿童、31例青年人、30例老年人双眼结膜囊取标本(取材前后称量棉拭运送管,差值即为标本质量)行细菌培养,阳性者进行细菌鉴定及定量计算:菌落数(colony forming units,CFUs)/标本质量。结果老年组结膜囊细菌检出率(93.33%)显著高于儿童组(45.45%)及青年组(50.00%),差异有统计学意义(P<0.01),且表皮葡萄球菌、类白喉棒状杆菌、金黄色葡萄球菌在老年组检出率均较高;老年组单眼共生细菌种类显著多于另外2组(P<0.01);儿童组结膜囊菌群数量明显少于青年组及老年组(P<0.01);类白喉棒状杆菌数量随年龄增长而增加,表皮葡萄球菌数量在老年组显著多于儿童组(P<0.01)。结论老年人结膜囊需氧菌种类、数量均高于青年及儿童,应该引起眼科医师重视。
Objective To investigate the types and numbers of aerobic conjunctival sacs of healthy people of different ages in Chongqing. Methods Wet cotton swabs were taken from 33 children, 31 young adults and 30 elderly patients with conjunctival sacs. The specimens were weighed before and after. Quantitative calculation: colony forming units (CFUs) / specimen mass. Results The detection rate of conjunctival bacteria (93.33%) in elderly group was significantly higher than that in children (45.45%) and young group (50.00%) (P <0.01). Staphylococcus epidermidis, (P <0.01). The number of conjunctival bacteria in children group was significantly less than that in young group and old group (P <0.01). The number of conjunctival bacteria in elderly group was significantly higher than that in other two groups <0.01). The number of Corynebacterium diphtheriae increased with age, and the number of Staphylococcus epidermidis in the elderly group was significantly higher than that in the children group (P <0.01). Conclusion The types and numbers of aerobic conjunctival sac in the elderly are higher than those of young children and children, and should be paid more attention by ophthalmologists.