论文部分内容阅读
目的研究清开灵注射液体外抗H1N1、H5N1、H7N9流感病毒的作用。方法以利巴韦林注射液作为阳性对照药物,通过测定细胞培养液上清血凝滴度(HA)和半数组织培养感染剂量(TCID50),评价清开灵注射液不同给药方式对H1N1、H5N1和H7N9流感病毒的抑制作用,并且通过MTT法测定清开灵注射液不同给药方式体外抗H1N1流感病毒作用。结果清开灵注射液半数中毒浓度(TC50)为62.5μL·m L-1;清开灵注射液有明显抑制流感病毒增殖的作用,以中和、治疗、预防的给药方式能显著降低H1N1、H5N1流感病毒的HA和TCID50,且以中和与治疗的给药方式作用最强,以治疗的给药方式能显著降低H7N9流感病毒的HA和TCID50;清开灵注射液以中和与治疗的给药方式体外抑制H1N1流感病毒的半数有效浓度(EC50)分别为4.07μL·m L-1和2.90μL·m L-1,治疗指数(TI)分别为15.36、21.55。结论清开灵注射液具有明显的体外抗H1N1、H5N1、H7N9流感病毒作用。
Objective To study the effect of Qingkailing injection against H1N1, H5N1 and H7N9 influenza viruses in vitro. Methods Ribavirin injection as a positive control drug was used to evaluate the effects of different dosage forms of Qingkailing Injection on the expression of H1N1, H5N1 and H7N9 influenza viruses, and MTT assay was used to determine the anti-H1N1 influenza virus in vitro by different administration methods of Qingkailing Injection. Results The median lethal concentration of Qingkailing injection (TC50) was 62.5 μL · m L-1. Qingkailing injection significantly inhibited the proliferation of influenza virus. The neutralizing, therapeutic and prophylactic administration could significantly reduce the H1N1 , H5N1 influenza virus HA and TCID50, and the most effective way of neutralization and treatment administration, the treatment can be administered to significantly reduce the H7N9 influenza virus HA and TCID50; Qingkailing injection for neutralization and treatment (EC50) were 4.07μL · m L-1 and 2.90μL · m L-1, respectively, and the therapeutic indices (TI) were 15.36 and 21.55 respectively. Conclusion Qingkailing injection has obvious anti-H1N1, H5N1, H7N9 influenza virus in vitro.