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以水溶性聚对苯撑乙炔分子刷(PPEB)为传感材料,荧光素标记的多肽为识别探针,设计了一种可实现肿瘤标志物快速、灵敏检测的蛋白质传感器。PPEB的刷状结构带有大量正电荷,与带负电荷的多肽形成静电复合物,使能量供体(PPEB)与受体(荧光素)之间的距离较近,发生高效荧光共振能量转移(FRET)。当多肽探针被前列腺特异性抗原(PSA)识别并切割为更小的寡肽片段时,由于肽段所带电荷量和等电点的变化,其与PPEB之间的静电作用减弱,能量供体和受体之间的距离增大,FRET效率降低。该传感器对PSA的检测可在40min内完成,具有良好的特异性和灵敏度。
Using water-soluble poly (p-phenylenevinylene) molecular brush (PPEB) as sensing material and fluorescein-labeled polypeptide as recognition probe, a protein sensor that can detect tumor markers rapidly and sensitively was designed. The brush-like structure of PPEB carries a large amount of positive charge and forms an electrostatic complex with the negatively charged polypeptide such that the distance between the energy donor (PPEB) and the receptor (fluorescein) is relatively close and efficient fluorescence resonance energy transfer occurs FRET). When the peptide probe is recognized by prostate specific antigen (PSA) and cleaved into smaller oligopeptide fragments, its electrostatic interaction with PPEB is weakened due to the change of the charge and isoelectric point carried by the peptide, and the energy supply The distance between the body and the receptor increases, and the FRET efficiency decreases. The sensor detection of PSA can be completed within 40min, with good specificity and sensitivity.