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【目的】观察两种环缩酚肽的衍生物(Hep-A)和(Hep-B)对氧诱导的血管增殖性视网膜病变的抑制作用。【方法】将鼠龄为7d(P7)的C57BL/6幼鼠置于体积分数75%的氧气箱中连续生活5d,建立氧诱导的血管增殖性视网膜病变模型。在12d(P12)幼鼠回到正常大气环境中,同时开始给小鼠皮下注安慰剂(第1组,n=23)、Hep-A10mg/kg(第2组,n=22)、或者Hep-B10mg/kg(第3组,n=22),每天两次,持续5d。在17d(P17)取鼠眼进行冰冻切片和GSA(griffoniasimplicifolialectinB4)染色,或作荧光素灌注和视网膜平铺片,用图象分析软件定量计算视网膜无灌注区和新生血管的面积。【结果】病理检测视网膜新生血管面积:第一组为(0.51±0.08)mm2/鼠(n=7);第2组为(0.11±0.01)mm2/鼠(n=8);第3组为(0.16±0.02)mm2/鼠(n=8)。视网膜平片检测视网膜新生血管面积:第1组为(1.36土0.02)mm2/眼(n=32),第2组为(0.19±0.01)mm2/眼(n=28),第3组为(0.07±0.01)mm2/眼(n=28);视网膜平片检测视网膜无灌注区面积:第1组为(2.33±0.08)mm2/眼,第2组为(1.08±0.09)mm2/眼,第3组为(1.22±0.11)mm2/眼。经ANOVA分析,第2组和第3组分别与第1组比较,视网膜新生血管的面积和视网膜无灌注区的面积,均明显减少,差异有统计学意义(P<0.01)。【结论】两种环缩酚肽衍生物均能强效?
【Objective】 To observe the inhibitory effect of Hep-A and Hep-B on oxygen-induced angioproliferative retinopathy. 【Method】 C57BL / 6 immature rats aged 7 days (P7) were placed in oxygen chamber with a volume fraction of 75% for 5 days. The oxygen-induced proliferative retinopathy model was established. At 12 d (P12), the pups returned to normal atmospheric conditions while the mice were started subcutaneously with placebo (Group 1, n = 23), Hep-A 10 mg / kg (Group 2, n = 22), or Hep -B 10 mg / kg (Group 3, n = 22) twice daily for 5 days. Murine eyes were harvested at 17d (P17) for frozen sections and graded with GSA (griffoniasimplicifolialectinB4) or fluorescein-perfusion and retinal patches. Image analysis software was used to quantify areas of the retinal nonperfusion and neovascularization area. 【Results】 The retinal neovascularization area was (0.51 ± 0.08) mm2 / mouse (n = 7) in the first group, (0.11 ± 0.01) mm2 / mouse in the second group (n = 8) (0.16 ± 0.02) mm2 / mouse (n = 8). Retinal plain film detected retinal neovascularization area: the first group was (1.36 ± 0.02) mm2 / eye (n = 32), the second group was (0.19 ± 0.01) mm2 / eye 0.07 ± 0.01) mm2 / eye (n = 28). The area of retinal nonperfusion area in the retinal plain film test was (2.33 ± 0.08) mm2 / eye in the first group and 1.08 ± 0.09 mm2 / eye in the second group The three groups were (1.22 ± 0.11) mm2 / eye. After ANOVA analysis, the area of retinal neovascularization and the area of retinal nonperfusion area were significantly reduced in group 2 and group 3 respectively compared with group 1, with statistical significance (P <0.01). 【Conclusion】 Both cyclic reductase derivatives can be potent?