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目的:测定淫羊藿黄酮类组分的表观溶解度和油水分配系数,为该组分整体水溶性和脂溶性的表征提供参考。方法:以淫羊藿黄酮类组分为模型药物,采用HPLC测定朝藿定A,B,C及淫羊藿苷在不同缓冲液中的平衡溶解度和表观油水分配系数(Papp),流动相乙腈-水(25∶75),检测波长270 nm。结果:朝藿定A,B,C和淫羊藿苷在不同pH缓冲液中的整体平衡溶解度顺序为朝藿定B>朝藿定A>朝藿定C>淫羊藿苷,Papp总体变化趋势均为先变大后变小再变大,油水分配系数-1.437~3.147。结论:淫羊藿黄酮类组分的水溶性及脂溶性较好,在不同pH缓冲液中变化趋势相近,朝藿定B属于微解,朝藿定A,C和淫羊藿苷属于极微溶解。
OBJECTIVE: To determine the apparent solubility and oil-water partition coefficient of Epimedium Flavonoids, providing references for the overall water-solubility and lipid-solubility characterization of this fraction. Methods: Epimedium flavonoids were used as model drugs. The equilibrium solubility, apparent oil-water partition coefficient (Papp) of epimedin A, B, C and icariin in different buffers were determined by HPLC. Acetonitrile-water (25:75), detection wavelength 270 nm. RESULTS: The overall equilibrium solubilities of epimedin A, B, C and icariin in different pH buffers were orderly: epimedin B> epimedin A> epimedin C> icariin, and the overall changes of Papp The trend is to first become larger and then smaller and then larger, oil and water distribution coefficient -1.437 ~ 3.147. CONCLUSION: Epimedium flavonoids have better water-solubility and liposolubility with similar trend in different pH buffers. Epimedin B belongs to microdissolution, epimedin A, C and icariin are minimal Dissolved.