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目的:探讨戊四氮慢性点燃癫痫对大鼠学习记忆能力的影响及海马神经颗粒素(neurogranin,Ng)的表达变化.方法:采用戊四氮(pentylenetetrazole,PTZ)腹腔注射慢性点燃癫痫(chronic epileptic,CEP)模型,用Morris水迷宫和Y迷宫对大鼠进行学习记忆能力检测,运用反转录多聚酶链反应(RT-PCR)方法和Western Blot方法分别测定大鼠海马NgmRNA和蛋白的水平变化.结果:与对照组比较,慢性癫痫发作组大鼠在水迷宫中的逃避潜伏期[(7.7±1.3)svs(4.5±1.1)s]延长(P<0.01),穿越平台次数[(5.7±2.1)vs(9.2±2.2)]减少(P<0.01),在Y迷宫中的错误反应次数[(12.8±2.5)vs(6.2±3.1)]增多(P<0.05),并伴有海马NgmRNA转录水平[(0.53±0.07)vs(0.74±0.04)]下降(P<0.01)和蛋白表达[(0.61±0.17)vs(0.91±0.23)]减少(P<0.05).结论:戊四氮慢性点燃癫痫大鼠学习记忆能力受损,海马Ng mRNA转录水平下降和蛋白表达减少可能参与了这一过程.
Objective: To investigate the effect of pentylenetetrazol (IGT) epilepsy on the learning and memory abilities of rats and the change of neurogranin (Ng) expression in hippocampus.Methods: The chronic epileptic rats were induced by pentylenetetrazole (PTZ) intraperitoneally , CEP) model. Morris water maze and Y maze were used to detect the learning and memory ability of rats. Reverse transcription polymerase chain reaction (RT-PCR) and Western Blot were used to determine the level of NgmRNA and protein in hippocampus of rats respectively. Results: Compared with the control group, the escape latency in water maze was significantly longer in the chronic epileptic seizure group (7.7 ± 1.3 vs 4.5 ± 1.1 s) (P <0.01) (12.8 ± 2.5) vs (6.2 ± 3.1)] (P <0.05), accompanied by a decrease of hippocampal Ng mRNA transcription level (P <0.01 vs vs (9.2 ± 2.2) (0.53 ± 0.07) vs (0.74 ± 0.04)] (P <0.01) and protein expression (0.61 ± 0.17 vs 0.91 ± 0.23) (P <0.05) .Conclusion: Pentylenetetrazole chronic ignition epilepsy Impaired learning and memory in rats, decreased hippocampal Ng mRNA transcription and decreased protein expression may be involved in this process.