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目的研究三七总皂苷(Panax notoginseng saponins,PNS)的小肠吸收动力学特性及生物黏附材料对三七总皂苷在小肠吸收速率常数和有效渗透系数的影响,考察生物黏附材料对三七总皂苷肠吸收的影响。方法高效液相色谱法测定灌流液中三七总皂苷的质量浓度,运用大鼠单向在体肠灌流模型考察三七总皂苷在十二指肠段与空-回肠段的吸收过程,以及生物黏附材料对三七总皂苷吸收过程的影响。结果在十二指肠段与空-回肠段中,药物质量浓度变化(0.5~2 mg·m L-1)对三七总皂苷的吸收速率常数和表观渗透系数无显著性差异(P>0.05);生物黏附材料(卡波姆934P与941P、HPMC K4M、壳聚糖和魔芋葡聚糖)能够提高三七总皂苷在小肠的吸收水平,尤其是在十二指肠段的吸收,而HPMC K15M促吸收效果不明显,果胶出现一定的吸收抑制现象。结论三七总皂苷在大鼠小肠中吸收较差,属于被动扩散,本实验所用生物黏附材料对三七总皂苷的肠吸收具有一定的促进作用。
Objective To study the intestinal absorption kinetics of Panax notoginseng saponins (PNS) and the effects of bioadhesive materials on the absorption rate constant and effective permeability coefficient of Panax notoginseng saponins in small intestine. To investigate the effect of bioadhesive materials on the absorption of Panax notoginseng saponins Absorption effects. Methods The concentration of Panax notoginseng saponins in perfusate was determined by high performance liquid chromatography (HPLC), and the absorption process of Panax notoginseng saponins in duodenum and empty ileum was investigated by the unilateral rat intestinal perfusion model. Effect of Adhesion Material on the Absorption of Panax Notoginseng Saponins. Results There were no significant differences in the absorption rate constants and apparent permeability coefficients of Panax notoginseng saponins between 0.5 and 2 mg · mL -1 in the duodenum segment and the ileum segment (P> 0.05). Bioadhesive materials (carbomer 934P and 941P, HPMC K4M, chitosan and konjac dextran) increased the absorption of Panax notoginseng in the small intestine, especially in the duodenum HPMC K15M promote absorption effect is not obvious, pectin absorption inhibition phenomenon. Conclusion Panax notoginseng has poor absorption in the small intestine of rats and belongs to passive diffusion. The bioadhesive materials used in this experiment may promote intestinal absorption of Panax notoginseng saponins.