论文部分内容阅读
[目的]探讨南方红豆杉根际微生物草木樨中华根瘤菌(Sinorhizobium meliloti)CHW10B的溶磷特性及促生作用。[方法]利用液体发酵试验比较不同时间、碳源、氮源、碳氮比、培养温度等条件下CHW10B菌株的溶磷量,采用温室盆栽法研究该菌株对南方红豆杉生长的影响,并分析该菌株产嗜铁素、精氨酸脱羧酶、ACC脱氨酶及有机酸等成分的能力。[结果]该菌株分别在培养时间4 d、磷酸钙5.00 g·L~(-1)、初始p H值7.0、装液量1/2、NaCl为0.0 g·L~(-1)、温度30℃、碳源为葡萄糖、氮源为硫酸铵、碳氮比100∶1时溶磷能力最强。将该菌株接种于1年生南方红豆杉实生苗360 d后,苗木的地径、苗高、生物量比对照分别增长了19.53%、20.14%、25.39%。该菌株能够产嗜铁素、精氨酸脱羧酶、ACC脱氨酶(0.922 U·mg~(-1))和IAA(8.908 mg·m L~(-1)),并可分泌大量有机酸—葡萄糖酸(5 704.92μg·L~(-1))。[结论]草木樨中华根瘤菌CHW10B溶磷能力强,对南方红豆杉促生作用显著,适用于多种不同酸碱性土壤,可高效应用于南方红豆杉的人工栽培,具有被开发为生物肥料的潜力。
[Objective] The research aimed to discuss the phosphorus-dissolving characteristics and promoting effect of Sinorhizobium meliloti CHW10B in the rhizosphere microbial community of Taxus chinensis. [Method] The liquid ferment experiment was used to compare the amount of dissolved phosphorus of CHW10B strain under different conditions of time, carbon source, nitrogen source, C / N ratio, incubation temperature and so on. The greenhouse pot experiment was conducted to study the effect of this strain on the growth of Taxus chinensis The strain produces ferritin, arginine decarboxylase, ACC deaminase and organic acids and other ingredients. [Result] The strain was cultured for 4 days, calcium phosphate 5.00 g · L -1, initial pH 7.0, liquid volume 1/2, NaCl 0.0 g · L -1, temperature 30 ℃, the carbon source is glucose, the source of nitrogen is ammonium sulfate, the ability of dissolving phosphorus is the strongest when the ratio of carbon to nitrogen is 100: 1. After inoculation of the strain for one year, the diameter, height and biomass of seedlings increased by 19.53%, 20.14% and 25.39%, respectively. The strain was able to produce a large number of organic acids such as endotoxin, arginine decarboxylase, ACC deaminase (0.922 U · mg -1) and IAA (8.908 mg · mL -1) - gluconic acid (5 704.92μg · L -1). [Conclusion] The A. tumefaciens CHW10B had strong ability of dissolving phosphorus, and had a significant promoting effect on the growth of Taxus chinensis var. Mairei. It was suitable for many kinds of acid-base soils and could be effectively used in the artificial cultivation of Taxus chinensis var. Mairei. potential.