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[目的]探讨对不同年龄段智力迟缓型小鼠予六味地黄丸干预后的智力水平的改变及其机制。[方法]将20只昆明雌性小白鼠数字随机化分为5组,每组4只,分别表示为A组(对照组);B组(肾虚组);C组(肾虚子代补肾组);D组(肾虚母代补肾组);E组(肾虚母、子代均补肾组)。随后将5只昆明雄性小白鼠随机投入上述5组中,雌雄交配后,取出雄鼠,对B、C、D、E组进行恐吓,并同时给予0.1%的三水醋酸铅水溶液,直到生产前,建立中医“恐伤肾”的模型。D、E组小鼠在正常孕育的同时予补肾干预。A、B、C组常规饲料及饮水。母鼠于交配后19~22d时先后产子,得到A、B、C、D、E组的子代鼠,C、E组的子代鼠饲料中继续添加六味地黄丸,A、B、D组子代鼠正常饮食,期间对不同年龄段的子代鼠分次进行行为学测试并分析。[结果]初生鼠翻身测试实验中补肾子代鼠的翻身成功率优于未补肾组,少年鼠空地测试显示补肾组在行为测试中的表现优于未补肾组,且长期补肾组(子代、母代鼠均补肾)优于只有子代或是母代补肾组,组间多重比较(LSD-t统计分析法)有统计学意义,A组与B组、C组、D组比较(t分别为27.50、8.10、9.30、3.00,P均﹤0.05),B组与C组、D组、E组比较(t分别为19.40、18.20、27.80,P均﹤0.05),C组与D组比较差异有统计学意义(t为8.40,P﹤0.05),D组与E组比较差异有统计学意义(t为9.60,P﹤0.05),青年鼠走钢丝测试,补肾组一定程度上仍优于未补肾组,A组与D组,B组与C组,B组与E组,C组与D组,D组与E组比较差异均有统计学意义(t分别为22.01、25.41、20.90、32.11、27.60,P均<0.05)。[结论]六味地黄丸对于改善智力迟缓型小鼠的智力水平具有一定的作用。
[Objective] To investigate the changes of intelligence level and its mechanism after intervention of Liuweidihuangwan in different mental retardation mice. [Methods] Twenty female Kunming mice were randomly divided into five groups (n = 4), which were represented as group A (control group), group B (group of kidney deficiency), group C Group D (Kidney Kidney Kidney Group); Group E (Kidney Kidney, Offspring Kidney group). Then, five Kunming male mice were randomized into the above five groups. After males and females mating, the male mice were taken out to intimidate the rats in groups B, C, D and E, and at the same time 0.1% aqueous lead tribasic acetate solution was given until the production. Establishment of traditional Chinese medicine “fear of kidney” model. D, E group of mice in normal pregnancy at the same time to kidney intervention. A, B, C group conventional feed and drinking water. The females gave birth to the progenies from 19 to 22 days after mating, and the progeny mice of groups A, B, C, D and E were added into the offspring rats of groups C and E to continue adding Liuweidihuangwan, groups A, B and D The offspring mice were given normal diet, during which the offspring of different age groups were tested in behavioral tests and analyzed. [Result] The turn-over success rate of kidney-tonifying rats was better than that of non-kidney-tonifying rats in the test of turning-over test in newborn rats. The open-air test in juvenile rats showed that the performance of the kidney- Mother rats were kidney) better than only the offspring or the mother’s kidney group, multiple comparisons between groups (LSD-t statistical analysis) were statistically significant, A group and B group, C group, D group (t respectively (P <0.05). There was a significant difference between group C and group D (P <0.05). The difference between group C and group D was statistically significant (t = 19.40, 18.20, 27.80, (T = 8.40, P <0.05). The difference between D group and E group was statistically significant (t = 9.60, P <0.05) Group A, group D, group B and group C, group B and group E, group C and group D, group D and group E had statistical significance (t = 22.01, 25.41, 20.90, 32.11, 27.60, P <0.05). [Conclusion] Liuweidihuang Pill has a certain effect on improving the intelligence level of retarded mice.