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目的探讨核受体共激活因子1(amplified-in-breast cancer 1,AIB1)在肝细胞肝癌中的表达及意义。方法肝细胞肝癌患者74例,取患者肝癌组织(肝癌组)和癌旁组织(癌旁组),采用免疫组织化学法检测2组AIB1蛋白阳性表达率,采用Western blot法检测2组AIB1蛋白表达相对量,分析AIB1阳性表达和肝细胞肝癌病理因素间关系。结果AIB1阳性表达产物主要分布于细胞质内,肝癌组AIB1阳性表达率(64.9%)明显高于癌旁组(40.5%),差异有统计学意义(P<0.05);肝癌组AIB1蛋白表达水平(105.68±4.21)明显高于癌旁组(7.57±0.29)(P<0.05);AIB1阳性表达在肿瘤直径、淋巴结转移及肿瘤远处转移上差异均有统计学意义(P<0.05)。结论 AIB1基因蛋白的过表达可能与肝细胞肝癌的发生、发展有关。
Objective To investigate the expression of AIB1 in hepatocellular carcinoma and its significance. Methods Totally 74 patients with hepatocellular carcinoma (HCC) were enrolled in this study. The positive rate of AIB1 expression in the two groups was detected by immunohistochemistry and the expression of AIB1 protein was detected by Western blot Relative amount, analyze the relationship between AIB1 positive expression and pathological factors of hepatocellular carcinoma. Results The positive expression of AIB1 was mainly localized in the cytoplasm. The positive expression rate of AIB1 in hepatocellular carcinoma (64.9%) was significantly higher than that in adjacent normal tissues (40.5%) (P <0.05) 105.68 ± 4.21) was significantly higher than that in adjacent noncancerous tissues (7.57 ± 0.29) (P <0.05). The difference of AIB1 expression in tumor diameter, lymph node metastasis and distant metastasis was significant (P <0.05). Conclusion The overexpression of AIB1 protein may be related to the occurrence and development of hepatocellular carcinoma.