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长链脂肪酰辅酶A合成酶(LACS,EC6.2.1)催化脂肪酸生成脂肪酰辅酶A,进入脂肪酸的β-氧化途径.本课题从拟南芥中克隆了LACS家族成员AtLACS1,构建表达载体,通过LiAc转化法转入酵母表达系统中表达,气质色谱(GC-MS)检测酵母细胞中脂肪酸组成.结果表明,拟南芥LACS1基因全长为1 983 bp,编码一个含660个氨基酸的多肽.以含空载酵母细胞中脂肪酸组成为对照,过表达LACS1的酵母细胞中棕榈一烯酸(16∶1)相对含量显著增加,硬脂酸(18∶0)的相对含量显著减少.这些结果提示,AtLACS1对脂肪酸代谢有一定的影响,能够活化游离的长链脂肪酸,尤其偏好C16和C18底物.“,”To enter the β-oxidation pathway,fatty acids need to be activated to form acyl-CoA catalyzed by acyl-CoA synthetase.Here we report the characterization of an A rabidopsis cDNA,which encodes a long chain fatty acyl-CoA synthetase (LACS,EC6.2.1.3) designated as LACS 1.AtLACS1 were cloned and the bioinformatics of both genes were analyzed.Meanwhile,expression vector was built and transformed into yeast (INVSC1) through LiAc method.Then gas chromatography (GC) was applied to detect the fatty acid composition of yeast cells.The sequence analyses revealed that AtLACS1 gene contained an open reading frame (ORF) of 1 983 bp,encoding a polypeptide of 660 amino acids.GC analysis showed that the level of palmitoleic acid (16∶1) in AtLACS1-transgenic yeast was increased and the content of stearic acid (18∶0) displayed an increasing tendency,compared with that in the control.This results demonstrated AtLACS1 could activate the free long chain fatty acids (C 16∶0 and C 18∶0) to form fatty acyl-CoA and involved in fatty acids metabolism.