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目的建立适用于电生理学研究具有良好可行性及可重复性的实验犬动眼神经损伤动物模型,探讨模拟动眼神经生物电活动的生理性电刺激参数。方法10只雌性Beagle犬常规开颅暴露动眼神经海绵窦后段,以不同强度电刺激动眼神经,采集下斜肌肌细胞复合动作电位后,将动眼神经锐性切断,再予直接端-端吻合。结果全部实验步骤均能被Beagle犬耐受,幕下段动眼神经充分暴露,刺激电极可顺利置入,并获得下斜肌理想的生物电信号。结论实验犬动眼神经损伤动物模型已经建立,并具有良好可重复性,为选择模拟动眼神经生物电活动的适宜刺激参数提供实验依据,以进一步用于日后动眼神经功能修复的电生理学研究。
Objective To establish an animal model of experimental oculomotor nerve damage which is suitable for electrophysiological study and to investigate the physiological parameters of electrical stimulation to simulate oculomotor bioelectrical activity. Methods Ten female Beagle dogs were exposed to the posterior segment of the oculomotor cavernous sinus by conventional craniotomy. The oculomotor nerve was electrostimulated with different intensities. After collecting the compound action potentials of the inferior oblique muscle cells, the oculomotor nerves were severed severely, - End anastomosis. Results All the experimental procedures were tolerated by Beagle dogs. The posterior subcutaneous ophthalmic nerve was fully exposed, and the stimulation electrodes could be inserted smoothly and the ideal bioelectrical signals of the inferior oblique muscles were obtained. Conclusion The animal model of oculomotor nerve damage in experimental dogs has been established and has good repeatability to provide experimental evidence for selecting appropriate stimulation parameters for the simulation of oculomotor bioenergetics and to further study the electrophysiological study of oculomotor nerve repair in the future .