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目的:观察三仁汤对大肠湿热证模型大鼠VitE、CGRP的影响,初步探讨三仁汤对大肠湿热证的作用机理。方法:将SD大白鼠45只,随机分为3组:空白对照组(A组)、大肠湿热证模型组(B组)、三仁汤治疗组(C组),每组15只,分笼饲养。除空白对照组外,其余各组均采用“高脂+高温高湿+大肠杆菌”的多因素复合造模方法,建立大肠湿热证动物模型。放免法检测大鼠血中VitE、CGRP的含量变化。结果:模型组大鼠血中VitE、CGRP含量明显下降,与空白对照组相比(P<0.05):治疗组经三仁汤治疗后,降低的血中VitE、CGRP逐渐升高恢复至正常水平,与大肠湿热证模型组相比(P<0.01),与空白对照组相比(P>0.05)。结论:大肠湿热证模型组大鼠血中VitE、CGRP明显降低,中药三仁汤能有效改善大肠湿热证模型大鼠诸症状,使降低的血VitE、CGRP增高恢复正常,提示其治疗机制可能是通过调节大鼠血中VitE、CGRP的含量起作用的。
Objective: To observe the effect of Sanren Decoction on VitE and CGRP in rats with damp-heat syndrome of the intestine, and to explore the mechanism of Sanren Decoction on damp-heat syndrome of the large intestine. Methods: Forty-five SD rats were randomly divided into three groups: blank control group (group A), large intestine damp-heat syndrome model group (group B) and Sanren decoction group (group C) Feeding. Except for the blank control group, the other groups adopted the multi-factor compound modeling method of “high fat + high temperature and high humidity + Escherichia coli” to establish animal models of damp-heat syndrome of the large intestine. Changes of serum levels of VitE and CGRP in rats by radioimmunoassay. Results: Compared with the blank control group, the levels of VitE and CGRP in the blood of model rats decreased significantly (P <0.05). After treatment with Sanren Decoction, the decreased blood levels of VitE and CGRP gradually returned to their normal levels , Compared with the model group of damp-heat syndrome (P <0.01), compared with the blank control group (P> 0.05). Conclusion: The levels of VitE and CGRP in the blood of rats with damp-heat syndrome of the large intestine were significantly decreased. The traditional Chinese medicine Sanren Decoction could effectively improve the symptoms of rats with damp-heat syndrome of the large intestine and make the decreased levels of serum VitE and CGRP return to normal, suggesting that the therapeutic mechanism may be Through regulating the content of VitE and CGRP in rat blood.