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[目的]探索恶性间皮瘤中高迁移率蛋白1(HMGB1)乙酰化修饰位点信息。[方法]利用Q Exactive四极杆—静电场轨道阱高分辨质谱和Proteome Discoverer软件,研究恶性间皮瘤细胞NCI-H2452中HMGB1的乙酰化位点,以肺癌细胞A549和卵巢癌细胞A2780作为参照。[结果]恶性间皮瘤细胞中HMGB1的较多Lysine发生乙酰化修饰,包括K43、K50、K57、K59、K60、K90、K136、K137、K167、K170,除K43和K90外,均为新报道的HMGB1乙酰化位点;然而在肺癌细胞和卵巢癌细胞中均未检测到HMGB1乙酰化修饰。[结论]本研究发现恶性间皮瘤中HMGB1存在特异性的乙酰化位点,其可能是潜在恶性间皮瘤诊断分子标志物。
[Objective] To explore the HMGB1 acetylation site information in malignant mesothelioma. [Method] The HMGB1 acetylation site in malignant mesothelioma cell NCI-H2452 was studied by Q Exactive quadrupole-electrostatic field orbit trap high resolution mass spectrometry and Proteome Discoverer software. The lung cancer cell A549 and ovarian cancer cell A2780 were used as a reference . [Results] The more Lysine of HMGB1 was acetylated in malignant mesothelioma cells, including K43, K50, K57, K59, K60, K90, K136, K137, K167 and K170, except for K43 and K90 Of the HMGB1 acetylation sites; however, no HMGB1 acetylation was detected in lung and ovarian cancer cells. [Conclusion] This study found that malignant mesothelioma HMGB1 specific acetylation sites, which may be potential molecular markers of malignant mesothelioma diagnosis.