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采用索氏提取法,超声提取法,回流提取法分别提取多序岩黄芪、天山岩黄芪根中总黄酮,并用荧光分析法进行含量测定。结果测得回流提取法提取的多序岩黄芪、天山岩黄芪根中总黄酮含量分别为12.005%,8.647%;索氏提取法提取的总黄酮含量分别为9.744%,7.348%;超声提取法提取的总黄酮含量分别为7.688%,5.840%。最后得出结论回流提取法是提取多序岩黄芪根中总黄酮的最佳方法。
Soxhlet extraction method, ultrasonic extraction method and reflux extraction method were used to extract total flavonoids from Astragalus membranaceus and Astragalus mongholicus roots respectively. The contents of total flavonoids were determined by fluorescence analysis. Results The content of total flavonoids in Radix Astragali and Astragalus mongolicus extracted by reflux extraction was 12.005% and 8.647%, respectively. The content of total flavonoids extracted by Soxhlet extraction was 9.744% and 7.348% respectively. The extraction by ultrasonic extraction The total flavonoids content was 7.688% and 5.840% respectively. Finally, it is concluded that reflux extraction is the best method to extract the total flavonoids from Astragalus membranaceus roots.