论文部分内容阅读
目的:研究山东地区肾上腺素能β3受体(β3-AR)基因多态性与冠心病及其危险因素的之间的关系。方法:对相互间无一级亲属关系的120例冠心病患者及114例非冠心病正常对照组人群,进行体重指数、腰臀围比、空腹血糖、血脂、血压、心电图、心脏彩超等检测,同时应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)核苷酸分型技术检测肾上腺素能β3受体基因及其基因多态性进行分析。结果:(1)冠心病组和正常对照组Trp64Trp、Trp64Arg和Arg64Arg的基因型频率分别为68.3%,30.0%,1.6%和69.2%,29.8%,0.9%。冠状动脉粥样硬化性心脏病组Arg等位基因频率(16.6%)与对照组(15.8%)频率近似(P>0.05),突变频率在冠心病与非冠心病之间相比无显著性差异。(2)多态性分布在冠状动脉粥样硬化性心脏病组不同冠脉病变支数亚组中也无显著性差异(x2=0.471,P=0.790)。结论:肾上腺素能β3受体基因Trp64Arg突变与山东地区人群冠心病的发生、发展无明显相关性。
Objective: To investigate the relationship between the polymorphism of adrenergic β3-receptor (β3-AR) gene in Shandong and coronary heart disease and its risk factors. Methods: The body mass index, waist-to-hip ratio, fasting blood glucose, blood lipid, blood pressure, electrocardiogram and echocardiography were detected in 120 CHD patients and 114 non-CHD normal control subjects without relatives. At the same time, the adrenergic β3 receptor gene and its gene polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) nucleotide typing. Results: (1) The genotype frequencies of Trp64Trp, Trp64Arg and Arg64Arg in coronary heart disease group and normal control group were 68.3%, 30.0%, 1.6% and 69.2%, 29.8% and 0.9% respectively. The frequency of Arg allele (16.6%) in coronary atherosclerotic heart disease group was similar to that in control group (15.8%) (P> 0.05), and there was no significant difference in the mutation frequency between coronary heart disease and non-coronary heart disease . (2) There was no significant difference in the distribution of polymorphism among the groups of coronary artery lesions with coronary atherosclerotic heart disease (x2 = 0.471, P = 0.790). Conclusion: The adrenergic β3 receptor gene Trp64Arg mutation and coronary heart disease in Shandong population no significant correlation between the development.