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目的探讨血小板生成素（Ｔｐｏ）受体Ｃｍｐｌ在急性白血病中的表达及其意义以及重组人Ｔｐｏ对急性白血病细胞增殖的影响。方法采用半定量逆转录聚合酶链反应（ＲＴＰＣＲ）方法检测４３例急性白血病患者骨髓单个核细胞Ｃｍｐｌ基因的表达，用ＭＴＴ法研究重组人Ｔｐｏ单独或联合某些造血生长因子对急性白血病细胞增殖的影响。结果３５例急性髓系白血病（ＡＭＬ）患者中有２２例表达Ｃｍｐｌ，各ＦＡＢ亚型均有表达。８例急性淋巴细胞白血病（ＡＬＬ）患者均无Ｃｍｐｌ表达。ＣＤ３４＋ＡＭＬ的Ｃｍｐｌ表达率（７２．７％）明显高于ＣＤ３４－组（３３．３％），Ｐ＜０．０５。Ｍ２ｂ、Ｍ３、Ｍ４ＥＯ的Ｃｍｐｌ表达率（４００％）明显低于其它ＡＭＬ亚型（８０．０％），Ｐ＜０．０５。Ｃｍｐｌ表达阳性组ＡＭＬ患者完全缓解率为７００％，Ｃｍｐｌ阴性组为８１．８％，两者比较，差异无显著性（Ｐ＝０．３９４）。２８例ＡＭＬ患者有９例白血病细胞体外经Ｔｐｏ作用后出现明显的增殖，其中１７例Ｃｍｐｌ表达阳性的病例有８例对Ｔｐｏ有反应，１１例Ｃｍｐｌ表达阴性的病例只有１例有反应，两组反应率比较，差异有显著性（Ｐ＜０．０５）。Ｔｐｏ体外对ＡＬＬ细胞的增?
Objective To investigate the expression and significance of thrombopoietin (Tpo) receptor Cmpl in acute leukemia and the effect of recombinant human Tpo on the proliferation of acute leukemia cells. Methods Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of Cmpl gene in bone marrow mononuclear cells in 43 patients with acute leukemia. MTT assay was used to study the effects of recombinant human Tpo alone or in combination with certain hematopoietic growth factors. The effect of cell proliferation in acute leukemia. RESULTS: C-mpl was expressed in 22 of 35 patients with acute myeloid leukemia (AML), and all FAB subtypes were expressed. None of the 8 acute lymphoblastic leukemia (ALL) patients had C-mpl expression. The C mpl expression rate of CD34+AML (72.7%) was significantly higher than that of the CD34-group (33.3%), P<0.05. The C-mpl expression rate of M2b, M3 and M4EO (400%) was significantly lower than that of other AML subtypes (80.0%), P<0.05. The complete response rate of AML patients in the C-mpl positive group was 70.0%, compared with 81.8% in the C-mpl negative group. There was no significant difference between the two groups (P=0.394). Nine out of 28 patients with AML had leukemia cells that showed significant proliferation in vitro after Tpo treatment. Of these, 17 cases with positive C mpl expression had response to Tpo in 8 cases, and only 11 cases with negative C mpl expression were found in 11 cases. The response, the two groups of reaction rates, the difference was significant (P <0.05). Tpo in vitro increase of ALL cells