乙烯利处理通过调节抗氧化酶活性和次生代谢产物的积累促进生姜根茎愈伤

• 作 者: 朱洁 李灿婴 孙磊 葛永红
• 机 构: 渤海大学食品科学与工程学院,辽宁 锦州 121013;生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁 锦州 121013
• 出 处: 食品科学
• 发表日期: 2022年5期
• 关键词: ethephon ginger rhizomes wound healing reactive oxygen species phenylalanine amm

生姜在收获、采后处理和运输过程中经常遭受机械损伤,已有报道称乙烯利能促进植物愈伤.本实验以生姜根茎为材料,研究采后乙烯利浸泡处理对其愈伤的影响及机制.结果表明,50 mg/L乙烯利处理能降低生姜根茎愈伤过程中的质量损失率,提高生姜根茎中过氧化氢、总酚和木质素含量.乙烯利处理还提高了生姜根茎中活性氧代谢相关酶(超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶)活性.乙烯利促进愈伤过程中酚类物质和木质素的积累与其提高过氧化物酶和苯丙氨酸解氨酶活性密切相关.由此说明,乙烯利处理主要通过调节活性氧代谢中关键酶活性和苯丙烷途径来提高抗氧化能力和次生代谢物质的积累,从而促进生姜根茎的愈伤.“,”Ginger rhizomes are subject to mechanical damage during harvest, handling, and transportation, and ethephon has been reported to promote plant wound healing. In this study, the effect and mechanism of ethephon treatment after harvest on wound healing in ginger rhizomes were investigated. The results indicated that 50 mg/L ethephon treatment retarded mass loss, and enhanced the contents of hydrogen peroxide, total phenolic compounds and lignin in ginger rhizomes during the wound healing process. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) involved in reactive oxygen species (ROS) metabolism were increased in the ethephon-treated ginger rhizomes. The accumulation of phenolic compounds and lignin during wound healing were correlated with a significant increase in peroxidase (POD) and phenylalanine ammonia lyase (PAL) activities. These findings suggest that ethephon treatment can promote the wound healing process of ginger rhizomes by modulating key enzyme activities involved in ROS metabolism and the phenylpropanoid pathway to enhance antioxidant capacity and accumulate secondary metabolites.