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以裸花紫珠丛生芽为外植体,在光照时间9h/d,光照强度1 500lx,温度(26±2)℃,pH 5.8的培养条件下,研究比较了不同无机盐量MS培养基、不同植物生长调节剂种类及配比、蔗糖浓度、培养周期等因素对裸花紫珠生长状况的影响,以期筛选出裸花紫珠微繁技术最佳增殖培养基。结果表明:丛生芽继代增殖的最佳配方为:MS+6-BA 2.0mg/L+NAA 0.05mg/L+蔗糖30g/L,30d的增殖系数达到10以上,丛生芽长势好。
In order to study the effects of different inorganic salts on MS culture medium with naked flower purple buds as explants under the conditions of illumination time 9h / d, light intensity 1500w, temperature 26 ± 2 ℃ and pH 5.8, The effects of different plant growth regulators, the ratio of sugar, sucrose concentration and culture period on the growth of Balsamoplankia were studied in order to screen out the best multiplication medium for micropropagation. The results showed that the optimal formula for the proliferation of clustered buds was MS + 6-BA 2.0mg / L + NAA 0.05mg / L + sucrose 30g / L, the multiplication coefficient of 30d reached above 10 and the buds grew well.