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为了解医院门诊部有关科室使用侵入性操作器械物品污染HBsAg的情况,某院于1998年10月～12月对相关的器械物品进行了HBsAg检测.1 材料与方法1.1 检测方法 采用ELISA查HBsAg,ELISA诊断试剂由上海实业科化生物技术有限公司生产,批号980920,失效期1999年3月2日(990302),操作步骤参照说明书,结果判断S/N值≥2.1为阳性.1.2 采样及样品处理 选择门诊开展侵入性操作的重点科室10个:包括耳鼻喉科、皮肤科、特诊科、消化科、泌尿外科、门诊部、急诊科、干部病房、普通外科.采样种类有:小壶喷头部、动态喉镜头部、口镜,换药室器械盘、镊子、剪刀、眼板镊、直止血钳,B超头,心电图导联头,胃镜、夹头,水槽、水龙头,纤维结肠镜、结肠镜头、夹头、镜管,膀胱镜镜头、管、夹头,检验采血窗口,麻醉喉镜,护士手等共计87份标本.用无菌棉试纸沾取0.01mol/L磷酸盐缓冲液(pH7. 4),在被检测物品上反复擦试10余次,放回试管采样液中(每管1ml),置4℃保存过夜,次日取出,3000r/min,10min,取上清液用ELISA检测HBsAg.
In order to understand the hospital outpatient departments use invasive surgical instruments of articles contaminated with HBsAg, a hospital in October to December 1998 on the relevant equipment items for HBsAg testing.1 Materials and methods 1.1 detection methods using ELISA to check HBsAg, ELISA diagnostic reagents produced by the Shanghai Industrial Branch of Biotechnology Co., Ltd., batch number 980920, the expiry date of March 2, 1999 (990302), steps refer to the instructions, the results determine the S / N value ≥ 2.1 positive .1.2 sampling and sample processing Select clinics to carry out invasive operations of key departments 10: including otolaryngology, dermatology, special medicine, gastroenterology, urology, outpatient department, emergency department, cadre ward, general surgery. Sampling types are: , Dynamic Laryngoscope Head, Ophthalmic Mirror, Dressing Room Instrument Tray, Tweezers, Scissors, Eye Tweezers, Straight Hemostatic Forceps, B Head, ECG Lead, Gastroscope, Chuck, Sink, Faucet, Fiber Colonoscopy, Colon A total of 87 specimens, including lens, collet, lens tube, cystoscope, tube, collet, blood sampling window, anesthesia laryngoscope and nurse hand, etc, were washed with 0.01mol / L phosphate buffer 4), was seized Wiping the article repeated more than 10 times, the sample was returned to the tube (1ml per tube), stored overnight at 4 ℃, removed the next day, 3000r / min, 10min, the supernatant was detected by ELISA HBsAg.