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光温敏不育系不育临界温度性状的稳定非常困难 ,其根本原因在于对这一性状的遗传模式缺乏了解。从粳型光敏不育系N5 0 88S中分离出仅存在临界温度差异的H5 0 88S株系 ,以两者为亲本 ,构建了包括正反交、回交及F2 分离群体在内的 7世代群体。将每粒谷苗一分为四而形成基因型构成相同的 4套材料 ,每套材料进行不同温度处理 ,以花粉育性为指标采用IECM算法进行遗传分析。结果表明 :四种温度条件下 ,正、反交F1育性差异不明显 ,表现低临界温度特性 ;F2 在不同温度下育性分离比例不同 ,2 3 5℃处理下低临界温度对高临界温度为显性 ;不育临界温度符合两对主效基因和微效基因共同作用的混合遗传模型 ,主效基因的遗传力为 80 %～ 97%。
It is very difficult to stabilize the critical temperature traits of photo-thermo-sensitive genic male sterile lines. The basic reason is that there is a lack of understanding of the genetic mode of this trait. The H5 0 88S line with only the difference of critical temperature was isolated from japonica CMS line N5 0 88S, and the 7-generation population including reciprocal crossing, backcrossing and F2 segregating populations . Each grain of seedling was divided into four to form four sets of materials with the same genotype, and each set of materials was treated at different temperatures. Genetic analysis was conducted using IECM algorithm based on pollen fertility. The results showed that under the four temperature conditions, there was no obvious difference in fertility between positive and negative F1s, which showed low-critical temperature. F2 had different fertility rates at different temperatures. Under the condition of 2350C, the effects of low-critical temperature on high-critical temperature Is dominant. The critical temperature of infertility is in line with the mixed genetic model of two major genes and minor genes. The heritability of main gene is 80% -97%.