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目的探讨幽门螺杆菌(Helicobacter pylori,HP)感染、微小核糖核酸-191(miR-191)表达与胃癌发生、发展的关系。方法采用HE和美蓝染色法检测HP在40例浅表性胃炎及40例胃癌组织中的感染情况;采用荧光原位杂交(fluorescence in situ hybridization,FISH)技术分别检测HP阳性和HP阴性胃炎组和胃癌组中miR-191的表达;分析HP感染、miR-191表达与胃癌临床病理特征的关系。结果 HP感染在胃炎组和胃癌组中的阳性率分别为30%、75%;两组相比差异有统计学意义(P<0.05)。FISH技术检测miR-191在胃炎组和胃癌组中阳性率分别为20%、62.5%,两组相比差异有统计学意义(P<0.05)。HP阳性胃炎组miR-191阳性率为33.3%:HP阴性胃炎组miR-191阳性率为7.1%;HP阳性胃癌组miR-191阳性率为73.3%,HP阴性胃癌组miR-191阳性率为50%。HP阳性组miR-191表达均高于同级别病变HP阴性组的水平,差异有统计学意义(P<0.05)。HP感染、miR-191表达与胃癌患者性别、年龄、肿瘤大小无明显相关性(P>0.05),与肿瘤分化程度、临床病理分期和淋巴结转移相关(P<0.05)。结论 HP感染和miR-191表达是导致胃癌发生、发展的重要因素,miR-191有望成为胃癌诊断和预后判断的客观指标之一。
Objective To investigate the relationship between Helicobacter pylori (HP) infection, the expression of miRNA-191 and the occurrence and development of gastric cancer. Methods HE and methylene blue staining were used to detect HP infection in 40 cases of superficial gastritis and 40 cases of gastric cancer. Fluorescence in situ hybridization (FISH) was used to detect HP positive and HP negative gastritis groups The expression of miR-191 in gastric cancer group was analyzed. The relationship between HP infection, miR-191 expression and clinicopathological features of gastric cancer was analyzed. Results The positive rates of HP infection in gastritis group and gastric cancer group were 30% and 75%, respectively. There was significant difference between the two groups (P <0.05). The positive rates of miR-191 detected by FISH in gastritis group and gastric cancer group were 20% and 62.5%, respectively. There was significant difference between the two groups (P <0.05). The positive rate of miR-191 in HP positive gastritis group was 33.3%: the positive rate of miR-191 in HP negative gastritis group was 7.1%; the positive rate of miR-191 in HP positive gastric cancer group was 73.3%; the positive rate of miR-191 in HP negative gastric cancer group was 50 %. The positive expression of miR-191 in HP positive group was higher than that in HP negative group in the same grade, the difference was statistically significant (P <0.05). HP infection. There was no significant correlation between the expression of miR-191 and the gender, age and tumor size (P> 0.05). The expression of miR-191 was correlated with tumor differentiation, clinicopathologic stage and lymph node metastasis (P <0.05). Conclusion HP infection and miR-191 expression are important factors that lead to the occurrence and development of gastric cancer. MiR-191 is expected to be one of the objective indicators for the diagnosis and prognosis of gastric cancer.