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目的探讨氢醌刺激对脐血单个细胞活性的影响及醌氧化还原酶(NQO1)、热休克蛋白10(HSP10)及巯氧还蛋白过氧化物酶(Prxs)的表达。方法取新鲜脐血分离单个核细胞,10μmol/L氢醌预刺激12 h后,再给予250μmol/L氢醌刺激5 h,磷脂蛋白/溴化丙啶(Anexin V/PI)染色检测细胞活力。荧光定量PCR检测NQO1、HSP10及Prxs的表达。结果脐血单个核细胞受到10μmol/L氢醌预刺激后,可耐受后续250μmol/L氢醌攻击,活细胞数从高剂量组的(44.92±25.10)%提高到(39.37±20.98)%,出现适应性反应。荧光定量PCR结果显示,与空白对照组比较,高剂量组HSP10、Prx3、Prx4表达分别增高(2.22±1.03),(3.44±1.07),(2.97±0.79)倍,适应组Prx1增高(1.08±0.29)倍。NQO1在高剂量组和适应组表达均较空白对照组增高(3.44±1.07)和(3.57±1.31)倍,Prx4在高剂量组表达增高(2.41±0.31)倍,且与适应组比较有明显差别。结论Prx1、Prx3、prx4、HSP10可能参与氢醌诱导的脐血单个核细胞适应性反应。
Objective To investigate the effect of hydroquinone on the activity of single cord blood cells and the expression of NQO1, HSP10 and Prxs. Methods Mononuclear cells were isolated from fresh umbilical cord blood. After being pre-stimulated with 10μmol / L hydroquinone for 12 hours, the cells were stimulated with 250μmol / L hydroquinone for 5 hours. Anexin V / PI staining was used to detect cell viability. Fluorescent quantitative PCR was used to detect the expression of NQO1, HSP10 and Prxs. Results After being pre-stimulated with 10μmol / L hydroquinone, mononuclear cells of cord blood could tolerate the subsequent 250μmol / L hydroquinone challenge. The number of viable cells increased from 44.92 ± 25.10% to 39.37 ± 20.98% Appear adaptive response. The result of real-time PCR showed that the expression of HSP10, Prx3 and Prx4 in the high-dose group increased (2.22 ± 1.03), (3.44 ± 1.07) and (2.97 ± 0.79) times compared with the blank control group ) Times. Compared with the control group, the expression of NQO1 in the high-dose group and the adaptive group was significantly higher (3.44 ± 1.07) and (3.57 ± 1.31) times than that in the blank control group, and the expression of Prx4 in the high-dose group was 2.41 ± 0.31 times . Conclusion Prx1, Prx3, prx4 and HSP10 may be involved in hydroquinone-induced cord blood mononuclear cell adaptive response.