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目的:探讨支原体单克隆抗体PD4在人喉癌不同组织中的表达及其临床意义。方法:采用免疫组织化学Envision法对256例喉不同病变组织标本(喉癌105例,颈部转移淋巴结31例,喉癌前病变18例,声带息肉53例,癌旁正常组织20例,癌周正常组织29例)进行PD4表达检测。结果:①人喉癌组织中PD4高表达率为44.76%(47/105),明显高于喉其他不同病变组织(P<0.01或P<0.05)。②中晚期(Ⅲ-Ⅳ期)、伴颈淋巴结转移、分化程度低(G3、G2)的喉癌病变组织中PD4表达阳性率明显高于早期(Ⅰ-Ⅱ期)、无颈淋巴结转移、分化程度高(G1)的喉癌组织(P<0.01或P<0.05)。③PD4表达阳性病例3、5年生存率明显低于PD4表达阴性病例(均P<0.01)。结论:喉癌的发生、发展及预后与支原体感染密切相关。
Objective: To investigate the expression of mycoplasma monoclonal antibody PD4 in different tissues of human laryngeal carcinoma and its clinical significance. Methods: Immunohistochemical Envision method was used to examine the pathological changes in 256 specimens of laryngeal tissues (105 cases of laryngeal carcinoma, 31 cases of cervical lymph node metastasis, 18 cases of precancerous lesions of larynx, 53 cases of vocal cord polyp, 20 cases of adjacent normal tissues, 29 cases of normal tissue) to detect PD4 expression. Results ① The high expression rate of PD4 in human laryngeal carcinoma was 44.76% (47/105), which was significantly higher than that in other laryngeal tissues (P <0.01 or P <0.05). ② The positive rate of PD4 expression in laryngeal lesions with advanced cervical lymph node metastasis and low differentiation (G3, G2) was significantly higher than that in early stage (stage Ⅰ-Ⅱ), without lymph node metastasis and differentiation (G1) laryngeal carcinoma (P <0.01 or P <0.05). The 3-year and 5-year survival rates of PD4-positive cases were significantly lower than those of PD4-negative cases (all P <0.01). Conclusion: The occurrence, development and prognosis of laryngeal carcinoma are closely related to mycoplasma infection.