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Enterovirus 71 is a neuroinvasive virus that is associated with severe neurological complications.We had earlier suggested that the replication capacity of a severe strain was higher than that of a mild strain.The recombinant 3CRV and 3CDRV virus strains were successfully rescued in our previous study.In the present study,we found no difference in virulence between 3CRV and severe strains.However,the capacity of replication and to cause cell injury of 3CDRV strain decreased in vitro,especially at 39.5℃.Replacement of 3CD region in the severe strain led to milder symptoms,less body weight loss,and lower viral load in ICR mice.Histopathological findings indicated less severe injury in mice infected with 3CDRV strain.This study suggests that the 3CD region contributes to the attenuation of the severe strain,including its replication capacity and temperature sensitivity.
Enterovirus 71 is a neuroinvasive virus that is associated with severe neurological complications. We had earlier suggested that the replication capacity of a severe strain was higher than that of a mild strain. Recombinant 3CRV and 3 CDRV virus strains were successfully rescued in our previous study. In the present study, we found no difference between virulence between 3CRV and severe strains. However, the capacity of replication and to cause cell injury of 3 CDRV strains decreased in vitro, especially at 39.5 ° C. Replacing of 3CD region in the severe strain led to Histopathological findings indicated less severe injury in mice infected with 3 CDRV strain. This study suggests that the 3CD region contributes to the attenuation of the severe strain, including its replication capacity and temperature sensitivity.