中研Ⅱ号对HI-SIV和HIVgp120抑制CD3/CD4信号转导通路的影响

• 作 者: 李平 关崇芬
• 机 构: 中国中医研究院基础理论研究所,中国中医研究院基础理论研究所 北京100700,北京100700
• 出 处: 北京中医药大学学报
• 发表日期: 2004年05期
• 关键词: 中研Ⅱ号 HIV-SIV HIVgp120 CD3/CD4

目的　研究中研II号治疗猴艾滋病的作用机理。方法　采用流式细胞仪检测法分析细胞周期 ;WesternBlot检测丝裂原活化蛋白激酶 (ERK2 )活性及电泳迁移率改变检测法 (EMSA)检测转录因子NF κB和AP 1活性。结果　对SIV感染猴模型 ,中研Ⅱ号可阻断HI SIV及HIVgp12 0介导的淋巴细胞功能 ,能抑制ERK2的活性 ,并降低转录因子NF κB和AP 1活性 ,调控HI SIV诱导的CD3/CD4 刺激信号转导通路。结论　中研Ⅱ号调节猴艾滋病T细胞功能可能是通过CD4 共刺激T细胞受体 (TCR)信号通路所致

Objective To study the mechanism of treatment of monkey AIDS by Zhongyan II. Methods Flow cytometry was used to analyze the cell cycle. Western Blot was used to detect the activity of mitogen-activated protein kinase (ERK2) and electrophoretic mobility shift assay (EMSA) was used to detect transcription factor NF κB and AP 1 activity. RESULTS: In the SIV-infected monkey model, Sinopharm II can block HI SIV and HIVgp12 0-mediated lymphocyte function, inhibit ERK2 activity, and reduce transcription factor NF κB and AP 1 activity, regulating HI SIV-induced CD3/CD4 Stimulate signal transduction pathways. Conclusion The regulation of monkey AIDS T cell function by Zhongyan No.2 may be caused by CD4 costimulatory T cell receptor (TCR) signaling pathway.