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目的:研究阿奇霉素与头孢噻肟对30株临床分离大肠杆菌的联合抗菌效应。方法:采用棋盘法设计,微量肉汤稀释法测定。测定不同浓度组合的2种抗菌药物对30株大肠杆菌的最低抑菌浓度,并计算FIC指数,以及观察两药不同加药顺序对效应的影响。结果:阿奇霉素与头孢噻肟联合应用后对大肠杆菌的浓度累积抑菌百分率曲线均比单独应用明显左移。FIC指数结果表明:阿奇霉素与头孢噻肟联合应用后对大肠杆菌17%为协同作用,60%相加作用,10%无关作用,13%拮抗作用;且拮抗作用均表现为于阿奇霉素或头孢噻肟的低于MIC的个别较低浓度处发生,而其他浓度处则仍表现为协同或相加作用。先后联用与同时应用抗菌效应差异无显著性(P>0.05)。结论:一般情况下,对临床分离大肠杆菌,阿奇霉素与头孢噻肟联合应用是合理的。
Objective: To study the combined antibacterial effect of azithromycin and cefotaxime against 30 clinical isolates of Escherichia coli. Methods: The checkerboard design, micro broth dilution method. The minimum inhibitory concentrations (MICs) of two antibacterials in different concentrations against 30 Escherichia coli strains were determined and the FIC index was calculated. The effects of different drug loading sequences on the effect were also observed. Results: The cumulative inhibitory percentage curves of E. coli after azithromycin combined with cefotaxime were all significantly shifted to the left as compared with the single application. FIC index results show that: azithromycin combined with cefotaxime 17% for Escherichia coli synergistic effect, 60% of the additive effect of 10% unrelated to 13% antagonistic effect; and the antagonistic effects were shown in azithromycin or cefotaxime Below the MIC at individual lower concentrations, while at other concentrations still appear to act synergistically or additively. There was no significant difference between antibacterial effect and antibacterial effect (P> 0.05). Conclusion: Under normal circumstances, the clinical isolation of E. coli, azithromycin and cefotaxime combined application is reasonable.