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人或动物的血氟含量与摄氟量密切相关,可直接反映机体氟水平,是相对稳定的生物标志物。但是,由于人或动物的正常血氟浓度处于痕量水平,加之现有氟化物测定技术仅能测定游离态氟,氟电极所测毫伏值E与氟离子浓度的对数(log C F)不呈能斯特响应,使得样品测定值偏差较大。国外研究者曾试用全固态离子电极,倒置平板式电极等方法测定微量血氟,效果尚可,但均需对现有装置作较大改制,难以普及应用。为此,本研究引入超声、生物酶消化、酸消化扩散法对血清样品进行系列化预处理,旨在破坏有机质,转化结合氟为易于测定的游离氟,从而提高血氟检出限。结果报告如下。
Human or animal blood fluoride content is closely related with fluoride exposure, which directly reflects the body’s fluorine level, is a relatively stable biomarker. However, since the normal blood fluoride level of a human or animal is at a trace level, and the existing fluoride determination technique can only determine the free fluorine, the logarithm (log CF) of the millivolt value measured by the fluorine electrode is not Nernst response, making the sample measured values deviate. Foreign researchers have tried all-solid-state ionic electrodes, inverted plate electrodes and other methods for the determination of trace blood fluoride, the effect is acceptable, but all need to make a larger system for the existing restructuring, it is difficult to universal application. Therefore, in this study, serum samples were subjected to serial pretreatment with sonication, enzymatic digestion and acid digestion and diffusion to destroy the organic matter and convert the bound fluorine to free fluorine, which is easy to measure, so as to improve the detection limit of blood fluoride. The results are reported below.