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目的通过与常用的外源性高脂饲料诱导高脂血症大鼠模型对比,探讨采用果糖灌胃法建立急性高甘油三酯血症大鼠模型的血脂水平及其动态变化趋势。方法选用SD雄性大鼠48只,随机分组,设对照组C1、C2、C3(分别为M1、M2、M3的对照组)均给予基础饲料喂养、模型组分别予高脂饲料(M1)、高脂乳糜剂(M2)、果糖(M3A、M3B、M4),于M1饲养4周,M2灌胃2周,M3果糖(M3A予8 mL/次,共5次;M3B予5 mL/次,共7次)。在末次灌胃0.5 h内,M4灌胃前、末次灌胃后8 h、16 h、24 h、36 h行眼球眦静脉取血,测大鼠总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL—C)、低密度脂蛋白胆固醇(LDL—C)含量。结果与对照组相比,高脂饲料及乳剂造模的M1、M2组TC显著升高(P<0.05),TG差异无统计学意义;果糖灌胃造模的M3A、M3B组TG显著升高(P<0.05),TC、HDL-C差别无统计学意义;M3A及M3B组间血脂水平无差异;M4组造模后TC、HLD-C随时间延长而逐渐下降;TG于灌胃后8 h达高峰,36 h恢复基础值。结论果糖灌胃模型可使TG急性稳定升高,不同果糖剂量和灌胃次数不影响造模效果,TG可持续升高36 h。
OBJECTIVE: To compare the blood lipid levels and trends of acute hypertriglyceridemia rats induced by fructose intragastrically with the common hyperlipidemia induced hyperlipidemia rat model. Methods Forty-eight male Sprague-Dawley rats were randomly divided into three groups: control group C1, C2 and C3 (control group M1, M2 and M3 respectively) were given basic diet. The model group was given high fat diet (M1) (M2A) and fructose (M3A, M3B and M4). The mice were fed with M1 for 4 weeks. M2 was orally administered for 2 weeks and M3 fructose (M3A was given to 8 mL / time for 5 times; M3B was given to 5 mL / 7 times). During the last intragastric administration of 0.5 h intramuscularly, the blood of the eyeballs was collected at 8 h, 16 h, 24 h and 36 h after the last intragastric administration of M4, and the levels of total cholesterol (TC), triglyceride (TG) , High-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) content. Results Compared with the control group, TCs in M1 and M2 groups were significantly increased (P <0.05), but there was no significant difference in TG between high-fat diet and emulsion model. TG in M3A and M3B group (P <0.05). There was no significant difference in TC and HDL-C between the two groups. There was no difference in serum lipid levels between M3A and M3B groups. TC and HLD-C in M4 group decreased gradually with time prolonging. h reached the peak, 36 h to restore the baseline value. Conclusion Fructose intragastric administration can elevate acute and stable TG, and different fructose doses and intragastric administration times do not affect the modeling effect. TG can be increased continuously for 36 hours.