论文部分内容阅读
目的观察异地子代成(新)螺、子代幼螺对日本血吸虫的易感性和存活情况,为血吸虫病监测预警(哨鼠疫水测定)和防治提供科学依据。方法在安徽安庆和江苏南京采集野外成(老)螺,在现场控制条件下自然繁殖,获取子代成(新)螺和幼螺,然后在室内人工感染无锡株日本血吸虫毛蚴(20条/只),并经饲养70d后分别解剖观察其感染情况及生存率。结果安庆和南京同龄成(老)螺的子代成(新)螺和幼螺毛蚴感染率分别为40.63%(91/224)、36.36%(88/242)和18.23%(33/181)、13.85%(27/195),差异均无统计学意义(χ2=0.892 92、1.346 41,P均>0.05);而两地子代成(新)螺与子代幼螺的感染率比较,则差异有统计学意义(χ2=29.568 3,P<0.01)。两地人工感染后的同龄子代成(新)螺和幼螺生存率分别为74.67%(224/300)、80.67%(242/300)和60.33%(181/300)、65.00%(195/300),差异均无统计学意义(χ2子代成螺=3.113 19、χ2子代幼螺=1.396 28,P均>0.05);但同地子代成(新)螺的存活率与子代幼螺比较差异有统计学意义(χ2安庆=14.047 5、χ2南京=18.607 1,P均<0.01)。结论安庆和南京两地子代成(新)螺及子代幼螺在实验室条件下均能感染无锡株日本血吸虫毛蚴,其感染率前者高于后者。因此,建议在现场开展哨螺测定时宜选择新鲜且活力强的成(新)螺为佳。
Objective To investigate the susceptibility and survival of Schistosoma japonicum in offspring of offspring of Achilles tendonensis and offspring, and to provide a scientific basis for the prevention and treatment of schistosomiasis surveillance and early warning. Methods The wild (old) snails were harvested in Anqing, Anhui Province and Nanjing, Jiangsu Province. They were naturally propagated under the control of the locus, and the offspring of Schistosoma japonicum mirabilis (20 pieces per bird ), And after 70 days of feeding respectively anatomical observation of their infection and survival rate. Results The infection rates of offspring from the same age of Anqing and Nanjing were 40.63% (91/224), 36.36% (88/242) and 18.23% (33/181) respectively, (Χ2 = 0.892 92,1.346 41, P> 0.05). However, compared with the infection rate of the offspring of the newborn snails and the offspring of the snails in the two regions, the difference was not statistically significant (13.85%, 27/195) The difference was statistically significant (χ2 = 29.5683, P <0.01). Survival rates of offspring of the same age offspring after artificial infection in both areas were 74.67% (224/300), 80.67% (242/300) and 60.33% (181/300), respectively, and 65.00% (195.00% 300). There was no significant difference between the two groups (chi2 progeny, = 3.113 19, chi2 progeny = 1.396 28, all P> 0.05) There were significant differences in juvenile snails (χ2 Anqing = 14.047 5, χ2 Nanjing = 18.607 1, P <0.01). Conclusion The offspring of Schistosoma japonicum and Schistosoma japonicum in both Anqing and Nanjing can infect miRNAs of Schistosoma japonicum in Wuxi strain under laboratory conditions, with the former being higher than the latter. Therefore, it is advisable to choose the fresh and strong (new) snail as the preferred test for the detection of snail in the field.