论文部分内容阅读
目的研究雌激素受体(ER)基因XbaI多态性与补钙对青春期少女骨骼发育作用的关系,为制定钙膳食参考摄入量提供科学依据。方法从志愿者中选取88名13~15岁青春期少女,随机分为补钙组和对照组,并按XbaI多态性分成不同的亚组,进行为期1年的补钙实验。在补钙前后测定全身各部位骨密度、骨钙素(BGP)、骨碱性磷酸酶(BAP)、抗酒石酸酸性磷酸酶(TRACP)、雌二醇(E2)及维生素D水平。比较不同基因亚型间补钙后骨密度增长或骨生化指标变化的差异。结果共72人完成了整个研究。经过1年的干预,补钙组BGP的增长及前臂远端1/10处的骨密度增长均大于对照组;补钙组内的Xx亚型反映骨形成的指标BAP的增长大于xx亚型,且全身各测量部位的骨密度增长值均大于xx亚型,但无显著性差异;而对照组内各XbaI亚型上述各指标的增长值未见统计学差异。结论XbaI多态性中Xx较xx亚型具有更好的补钙反应性,在今后的补钙干预中应考虑该遗传因素的影响。
Objective To study the relationship between the XbaI polymorphism of estrogen receptor (ER) gene and the bone development of adolescent girls with calcium supplementation, and provide a scientific basis for formulating reference intake of calcium diet. Methods Eighty-eight adolescent girls aged 13-15 years were selected from volunteers and randomly divided into calcium-supplemented group and control group. According to the XbaI polymorphism, they were divided into different subgroups for one-year calcium supplementation. Bone mineral density (BMD), BGP, BAP, TRACP, E2 and vitamin D were measured before and after calcium supplementation. Differences in bone mineral density (BMD) or changes in bone biochemical markers after calcium supplementation were compared between different genotypes. A total of 72 people completed the entire study. After one year of intervention, the growth of BGP in the calcium group and the bone density in the distal part of the forearm increased more than that of the control group. The Xx subtype in the calcium group showed that the increase of BAP in the bone formation was larger than that in the xx subtype, And the increase of BMD in all the measurement sites was greater than that of xx subtype, but no significant difference was found. However, the growth of each index of XbaI subtype in the control group showed no significant difference. Conclusions Xx has a better calcium reactivity than xx subtype in XbaI polymorphism. The influence of this genetic factor should be considered in future calcium intervention.