论文部分内容阅读
目的 :探讨补充微量营养素对HIV感染者及AIDS病人免疫功能的影响。方法 :100例HIV感染者和100例AIDS病人分别随机分为研究组和对照组,每组50例。采用双盲法,研究组给予微量营养素片,1片/d,持续口服6个月。对照组给予安慰剂片,1片/d,持续口服6个月。各组干预前、干预6个月后分别采集静脉血,经全自动生化分析仪检测全血总蛋白、白蛋白水平;原子吸收分光光度法检测血清镁、锌、铁、钙水平;流式细胞检测仪检测血中CD_3~+、CD_4~+、CD_8~+T淋巴细胞;ELISA法检测血中IgA、IgG、IgM水平。结果 :HIV感染者干预后血浆总蛋白、白蛋白水平均明显高于干预前。HIV感染者研究组干预后血清锌、钙、镁、铁含量均明显高于干预前及对照组;研究组干预后血中CD3+、CD4+、CD8+T淋巴细胞水平明显高于干预前及对照组干预后。AIDS患者研究组干预后血清锌、钙、镁、铁含量均明显高于干预前及对照组干预后;研究组与对照组干预前后血中CD_3~+、CD_4~+、CD_8~+T淋巴细胞、IgA、IgG、IgM水平均无统计学差异。结论 :通过补充微量营养素,能明显提高HIV感染者及AIDS患者血中微量元素水平;可明显提高HIV感染者血中免疫细胞数量及血浆总蛋白、白蛋白水平。但是对AIDS患者免疫功能、血浆总蛋白、白蛋白水平则无显著影响。
Objective: To investigate the effect of micronutrient supplementation on immune function in HIV-infected and AIDS patients. Methods: 100 HIV-infected patients and 100 AIDS patients were randomly divided into study group and control group, 50 cases in each group. Double-blind method, the study group was given micronutrient tablets, 1 / d, sustained oral administration of 6 months. The control group was given placebo tablets, 1 / d, sustained oral administration of 6 months. Venous blood was collected before intervention and 6 months after intervention. Total protein and albumin in whole blood were detected by automatic biochemical analyzer. Levels of serum magnesium, zinc, iron and calcium were measured by atomic absorption spectrophotometry. Flow cytometry Detector detected CD_3 ~ +, CD_4 ~ +, CD_8 ~ + T lymphocytes in blood and IgA, IgG, IgM levels in blood by ELISA. Results: The levels of plasma total protein and albumin in HIV-infected patients were significantly higher than those before intervention. The serum levels of Zinc, Calcium, Magnesium and Iron in the HIV-infected study group were significantly higher than those before the intervention and in the control group. The levels of CD3 +, CD4 + and CD8 + T lymphocytes in the study group were significantly higher than those before the intervention and in the control group After intervention The levels of serum zinc, calcium, magnesium and iron were significantly higher in the study group of AIDS patients than those in the control group before and after the intervention; the levels of CD_3 ~ +, CD_4 ~ +, CD_8 ~ + T lymphocytes in the study group and the control group before and after intervention , IgA, IgG, IgM levels were not statistically different. Conclusion: By supplementing micronutrients, the blood levels of trace elements in HIV-infected and AIDS patients can be significantly increased, and the number of immune cells and total protein and albumin in blood of HIV-infected persons can be significantly increased. However, immune function in patients with AIDS, plasma total protein, albumin levels had no significant effect.