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目的:研究南海红树林内生真菌SK7RN3G1的代谢产物。方法:采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定其化学结构。结果:从南海红树林内生真菌SK7RN3G1的菌体中分离得到6个代谢产物。经波谱解析,分别为:3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate(1),lichenxanthone(2),griseoxanthone C(3),1,3,6-trihydroxy-8-methyl-9H-xanthen-9-one(4),harman(1-甲基-β-咔啉)(5),N9-甲基-1-甲基-β-咔啉(6)。初步药理活性显示化合物1和2对人体肝癌细胞HepG2抑制的IC50分别为20,25 mg.L-1。根据文献报道,化合物5具有抗HIV活性,其EC50为10.7μmol.L-1,化合物6具有抗HIV和抗癌重要生理活性。结论:所有化合物均首次从南海红树林内生真菌SK7RN3G1中分离得到。
Objective: To study the metabolites of endophytic fungi SK7RN3G1 in the South China Sea. Methods: The compounds were isolated and purified by repeated silica gel column chromatography and Sephadex LH-20 gel chromatography. Their chemical structures were identified by physico-chemical constants and spectral analysis. Results: Six metabolites were isolated from the endophytic fungus SK7RN3G1 in the South China Sea. The results of spectral analysis showed that 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1- carboxylate (1), lichenxanthone (2), griseoxanthone C -8-methyl-9H-xanthen-9-one (4), harman (5), N9-methyl-1-methyl-β-carboline (6). The preliminary pharmacological activities showed that the IC50s of compounds 1 and 2 on human HepG2 cells were 20 and 25 mg.L-1, respectively. According to reports in the literature, compound 5 has anti-HIV activity with EC50 of 10.7 μmol.L-1, and compound 6 has anti-HIV and anti-cancer important physiological activity. Conclusion: All the compounds were isolated from the endophytic fungus SK7RN3G1 in the South China Sea for the first time.