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从麻风杆菌和鸟分枝杆菌中提取的35k Da蛋白是预防麻风病的亚单位疫苗的一种理想抗原。作者从质粒 p LJ3中扩增麻风杆菌 35k Da蛋白编码基因 ,将此基因克隆至p JW430 3( DNA- Neg)中 ,用标准分子生物学技术和 35k Da特定引物构建质粒 p ILM35( DNA- 35)。用 1 0 0 μg DNA- 35?
The 35k Da protein extracted from Mycobacterium leprae and Mycobacterium avium is an ideal antigen for the prevention of leprosy subunit vaccines. The gene was cloned from plasmid pLJ3 and cloned into pJW4303 (DNA-Neg). The plasmid pLM35 (DNA-35) was constructed using standard molecular biology techniques and 35 kDa specific primers ). With 100 μg DNA-35?