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建立了羊肉组织中胺菊酯和三氟氯氰菊酯的固相萃取-反相高效液相色谱测定法。采用氟罗里硅土固相萃取柱(1000 mg/6 mL)进行固相萃取。以Shim-pack VP-ODS(200 mm×4.6 mm)柱为分析柱,流动相为甲醇:水=95:5(V/V),流速为0.7 mL/min。胺菊酯和三氟氯氰菊酯分别在0.01～6.40μg/mL(r=0.9999)和0.068～7.20ug/mL(r=0.9998)范围内与峰面积呈良好线性关系,检出限分别为0.001μg/mL和0.002μg/mL,胺菊酯和三氟氯氰菊酯的回收率为90.2%～101.4%,相对标准偏差为2.3%～4.0%。该方法可作为羊肉组织中胺菊酯和三氟氯氰菊酯含量监测的控制方法。
A solid-phase extraction-RP-HPLC method was established for the determination of tetramethrin and cyhalothrin in lamb tissues. Solid-phase extraction was performed using Florisil silica SPE (1000 mg / 6 mL). The analytical column was Shim-pack VP-ODS (200 mm × 4.6 mm). The mobile phase was methanol: water = 95: 5 (V / V) and the flow rate was 0.7 mL / min. Tetramethrin and cyhalothrin had a good linear relationship with the peak area in the range of 0.01-6.40μg / mL (r = 0.9999) and 0.068-7.70μg / mL (r = 0.9998) with the detection limits of 0.001μg / mL and 0.002 μg / mL respectively. The recoveries of tetramethrin and cyhalothrin were 90.2% -101.4% with relative standard deviations of 2.3% -4.0%. The method can be used as a control method for monitoring the contents of tetramethrin and cyhalothrin in mutton tissues.