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本文在顺灌（由主动脉根部灌）冷钾氧合稀释血实施换瓣术条件下，对１０例风心病患者心房肌线粒体变化做了动态定量研究，体外循环条件降温至３０℃，灌注液４℃。结果表明，在停跳开始（时相Ｉ）至缺血４０分钟（时相Ⅱ），线粒体损伤主要是Ｉ级，而含钙粒线粒体及所含钙粒的Ｖｖ（体密度）增长不显著。复跳期（时相Ⅲ），Ⅱ级损伤病例增至５０％，含钙粒线粒体及所含钙粒的Ｖｖ剧增，较时相Ⅱ分别增加９４％和８２％。这可能是瞬间血流再灌注导致的损伤，显示经历８１分钟（平均）缺血的线粒体已有钙超载及再灌注损伤的异常反应性。在本文条件下，估计心肺旁路最佳时间为４０～５０分钟，最大耐受时间在８０分钟以内，有些病例术后心功恢复不佳，大概与顺灌不全或术时过长有关。
In this paper, cis-perfusion (by the aortic root irrigation) cold potassium oxygenated diluted blood under the condition of valve replacement, 10 patients with rheumatic heart mitochondrial changes in atrial and dynamic quantitative study of cardiopulmonary bypass conditions cooling to 30 ℃, perfusion fluid 4 ° C. The results showed that mitochondrial injury was mainly grade I at the beginning of arrest (phase I) and 40 minutes after ischemia (phase II), while Vv (body density) increase of calcium-containing and calcium-containing granules was insignificant. During the recovery phase (phase Ⅲ), the grade Ⅱ injury increased to 50%. The Vv of calcium-containing mitochondria and calcium-containing granules increased sharply by 94% and 82% respectively compared with that of phase Ⅱ. This may be due to transient blood flow reperfusion injury, showing that the mitochondria that have undergone 81 minutes (mean) ischemia have been found to have abnormal calcium reactivity and reperfusion injury. Under this condition, the optimal time for cardiopulmonary bypass is estimated to be 40 to 50 minutes and the maximum tolerated time is within 80 minutes. In some cases, postoperative heart function recovery is poor, which is probably related to incomplete irrigation or prolonged operation.