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Dane等应用免疫电子显微镜技术发现,在一些急性的乙型肝炎抗原阳性的肝炎患者血清中,有特殊的双壳的球状病毒颗粒(Dane颗粒),直径为42毫微米,并存在着另一个抗原抗体系统。高速离心全血清所得到的抗原颗粒用洗涤剂处理后,Dane颗粒被分成有乙型肝炎抗原活性的外壳和直径为27毫微米、形态与肠道病毒类似的内部成分或核心。乙型肝炎患者恢复期血清中的抗体与核心起反应,产生免疫聚集体。这种核心抗体(anti-HBc)与外壳抗体(anti-HBs,外壳抗原即HBsAg)的特异性完全不同。Hoofnagle等进一步证明所有HBsAg
Dane and other applications of immuno-electron microscopy found that in some patients with acute hepatitis B antigen-positive hepatitis serum has a special bivalve globular virus particles (Dane particles), a diameter of 42 nm, and the presence of another antigen Antibody system. After the antigen particles obtained from the high-speed centrifugation of the whole serum were treated with the detergent, the Dane particles were separated into a hepatitis B antigen-active shell and a 27-nm-diameter internal component or core similar to enterovirus. Antibodies in the serum of patients with convalescent hepatitis B react with the nucleus to produce immune aggregates. The specificity of this core antibody (anti-HBc) is different from that of the anti-HBs (HBsAg). Hoofnagle et al further prove that all HBsAg