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目的：建立 HPLC 法测定复方依普罗沙坦/氢氯噻嗪片含量的方法。方法：采用菲罗门 C18色谱柱(250 mm ×4.6 mm,5μm),以0.5%甲酸水溶液-乙腈(60∶40, pH 2.80)为流动相,流速1.0 ml·min-1,检测波长272 nm,柱温30℃,进样量20μl。结果：依普罗沙坦质量浓度在60.0~1200.0 mg·L-1范围内(r=0.9999),氢氯噻嗪质量浓度在1.25~25.00 mg· L-1范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率分别为100.02%(RSD=0.35%,n=9)、97.93%(RSD=1.54%, n=9)。结论：本方法简便、快速、准确,重复性好,可用于复方依普罗沙坦/氢氯噻嗪片中两组分的含量测定。“,”Objective:To establish an HPLC method for the simultaneous determination of eprosartan/hydrochlorothiazide tablets. Methods:Isocratic separation was achieved on a Phenomenex C18 column(250 mm × 4. 6 mm, 5 μm) using the mobile phase com-posed of 0. 5% formic acid-acetonitrile(60∶40, pH 2. 80). The flow rate was 1. 0 ml·min-1, the detection wavelength was 272nm, the column temperature was 30℃ and the injection volume was 20μl. Results:The linearity between peak area and concentration was observed within the range of 60. 0-1 200. 0 mg·L-1(r=0. 999 9) for eprosartan and 1. 25-25. 00 mg·L-1(r=0. 999 9) for hydro-chlorothiazide. The mean recovery of eprosartan and hydrochlorothiazide was 100. 02%(RSD=0. 35%, n=9) and 97. 93%(RSD=1. 54%, n=9), respectively. Conclusion:The method is simple, sensitive and accurate, and can be applied in the determination of eprosartan/hydrochlorothiazide tablets.