论文部分内容阅读
目的探讨苯并咪唑对雄性大鼠生育力的影响及其作用靶点。方法将100只健康清洁级Wistar雄性大鼠随机分为4组,分别为低(20 mg/kg)、中(100 mg/kg)、高剂量(200 mg/kg)苯并咪唑染毒组和对照(0.5%吐温80溶液)组,每组25只。采用经口灌胃染毒方式进行染毒,染毒容量为10 ml/kg,每天1次,连续染毒80 d。染毒结束后,雌、雄大鼠以1∶1合笼,观察雌性大鼠妊娠情况,并计算雄性大鼠的交配率和生育力指数。测定精子活动率和精子数量及血清中促黄体生成素(LH)、卵泡刺激素(FSH)和睾酮(T)水平,采用流式细胞学术测定睾丸细胞周期的情况。结果与对照组比较,中、高剂量苯并咪唑染毒组雄性大鼠的交配率和G2期/M期的比例显著升高,生育力指数、精子活动率和精子数量以及睾丸细胞G0期/G1期及S期的比例显著降低,差异均有统计学意义(P<0.05,P<0.01);且随着苯并咪唑染毒剂量的升高,雄性大鼠的交配率和G2期/M期的比例均呈上升趋势,生育力指数、精子活动率和精子数量以及睾丸细胞G0期/G1期及S期的比例均呈下降趋势。与对照组比较,仅高剂量苯并咪唑染毒组雄性大鼠血清中LH水平显著降低,差异有统计学意义(P<0.05),且雄性大鼠血清中LH水平随着苯并咪唑染毒剂量的升高而下降;而各剂量苯并咪唑染毒组雄性大鼠血清中FSH、T水平均无显著变化。结论苯并咪唑对雄性大鼠具有明显的生殖毒性,可引起精子生成障碍,导致雄性大鼠生育力降低,甚至不育。
Objective To investigate the effect of benzimidazole on the fertility of male rats and its target of action. Methods 100 healthy male Wistar rats were randomly divided into 4 groups: low (20 mg / kg), medium (100 mg / kg) and high dose (200 mg / kg) Control (0.5% Tween 80 solution) group, 25 rats in each group. Using oral gavage exposure method, the exposure capacity of 10 ml / kg, 1 times a day, continuous exposure to 80 d. After the exposure, female and male rats were caged 1: 1 to observe the pregnancy of female rats. The mating rate and fertility index of male rats were calculated. The activity of sperm and the number of sperm and serum levels of LH, FSH and T were measured. The testicular cell cycle was measured by flow cytometry. Results Compared with the control group, the mating rate and the ratio of G2 phase / M phase in male and female rats exposed to benzimidazole were significantly increased. The fertility index, sperm motility, sperm count and testicular cell G0 phase / G1 phase and S phase were significantly decreased (P <0.05, P <0.01). And with the increase of benzimidazole dose, the mating rate of male rats and the ratio of G2 / M The proportion of fertility index, sperm motility and sperm count, as well as the ratio of G0 / G1 phase and S phase in testicular cells all showed a decreasing trend. Compared with the control group, only LH levels in the male rats exposed to high doses of benzimidazole were significantly decreased (P <0.05), and the levels of LH in the serum of male rats were significantly lower than those of the control The dose increased and decreased; however, the levels of FSH and T in serum of male rats in each dose of benzimidazole did not change significantly. Conclusions Benzimidazole has obvious reproductive toxicity to male rats, which can cause obstacles to spermatogenesis and lead to decreased fertility and even infertility in male rats.